Nunuk Dyah Retno Lastuti, - and Endang Suprihati, - and Dony Chrismanto, - and Anwar Ma’ruf, - (2018) Antigenic Protein of Leucocytozoon caulleryi schizont Inducing Cellular Immune Resonse: TLR-2 and CD4 as Marker. Advances in Health Sciences Research; 1st International Conference in One Health (ICOH 2017), 5. pp. 67-70. ISSN 978-94-6252-481-1

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Abstract

Leucocytozoonosis is caused by Leucocytozoon caulleryi and is responsible for death in chickens by bleeding. Leucocytozoonosis is an endemic disease in Indonesia and incidences have been reported in several regions in East and Central Java. The financial losses caused by this disease include growth disorders in chick, decreased egg production, higher mortality rate and also a higher production cost. This research aims to detect TLR-2 and CD4 expression as a cellular immune response in rabbits immunised by the protein of L.caulleryi schizont. It is needed as a preliminary research study for molecular vaccine development which is considerably effective when it comes to preventing leucocytozoonosis occurance in Indonesia. This research study was performed in several stages i.e. the isolation of L.caulleryi schizont from a chicken liver infected with leucocytozoonosis to be based on the clinical signs observed, microscopic examination, and the pathological changes in the other chicken organs. The purification of the soluble protein of L.caulleryi schizont including the immunisation of the rabbits. Each of the experimental rabbits was injected with 500 μg of L.caulleryi schizont protein and added adjuvant complete with a ratio of 1:1. Every two weeks the injection was performed with the same protein with a dosage of 500 μg each and an added adjuvant that was incomplete (the booster was performed 5 times in 2 weeks). The examination of the cellular immune response of CD4 and TLR-2 expression in the rabbits’ T cells using an immunocytochemistry method visualised by fluorescein isothiocyanate. The examination of the results was done by immunocytochemistry showing TLR-2 and CD4 expression as yellow to green fluorescent colour, mainly in the 5th booster where the activation of the CD4 coreceptor and TLR-2 occurred. The conclusion shows that the antigenic protein of L.caulleryi schizont has the ability to induce a cellular immune response through the co-receptors CD4 and TLR-2 in the rabbits’ T cells as the preliminary research in the sub-unit vaccine development for leucocytozoonosis in chickens. KEY WORD: antigenic protein, Leucocytozoon caulleryi, schizont, TLR-2, CD4

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