Reina Puspita Rahmaniar and Maya Nurwantanti Yunita and Mustofa Helmi Effendi and Sheila Marty Yanestria (2020) Encoding Gene for Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Nasal Swab of Dogs. The Indian Veterinary Journal, 97 (2). pp. 37-40. ISSN 0019 - 6479, E - ISSN 0974 - 9365

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Abstract

The purpose of this study was to isolate and identify encoding gene for methicillin-resistant Staphylococcus aureus (MRSA) from nasal swab of dogs in Surabaya, Indonesia. Nasal swab of dogs of 85 samples obtained from five areas in Surabaya. Bacterial identification was based on the growth in Mannitol Salt Agar, Gram staining, catalase, coagulase and VP tests. 43 (50.59%) out of 85 samples were for positive Staphylococcus aureus isolation. MRSA confirmation by Oxacillin Resistant Screen Agar Base (ORSAB) were 25(29.41%). The molecular identification on mecA gene by PCR showed that 5(5.88%) isolates were positive contain mecA gene. It was concluded that the dogs as companion animals can be a potential reservoir for MRSA strains to threat public health Key words: Staphylococcus aureus, MRSA, Dogs, mecA gene

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