Pengaruh Konsentrasi Polimer Alginat Dan Konsentrasi Larutan Sambung Silang Bacl2 Terhadap Karakteristik Mikrosfer Ovalbumin-Alginat(Dibuat dengan Metode Ionotropic Gelation Teknik Aerosolisasi)

Mukhlis Bayu Irawan, - (2015) Pengaruh Konsentrasi Polimer Alginat Dan Konsentrasi Larutan Sambung Silang Bacl2 Terhadap Karakteristik Mikrosfer Ovalbumin-Alginat(Dibuat dengan Metode Ionotropic Gelation Teknik Aerosolisasi). Skripsi thesis, UNIVERSITAS AIRLANGGA.

[img] Text (HALAMAN JUDUL)
1. HALAMAN JUDUL .pdf

Download (387kB)
[img] Text (ABSTRAK)
2. ABSTRAK .pdf

Download (257kB)
[img] Text (DAFTAR ISI)
3. DAFTAR ISI .pdf

Download (272kB)
[img] Text (BAB I PENDAHULUAN)
4. BAB I PENDAHULUAN.pdf

Download (263kB)
[img] Text (BAB II TINJAUAN PUSTAKA)
5. BAB II TINJAUAN PUSTAKA.pdf
Restricted to Registered users only until 21 January 2023.

Download (722kB) | Request a copy
[img] Text (BAB III KERANGKA KONSEPTUAL)
6. BAB III KERANGKA KONSEPTUAL.pdf
Restricted to Registered users only until 21 January 2023.

Download (259kB) | Request a copy
[img] Text (BAB IV METODE PENELITIAN)
7. BAB IV METODE PENELITIAN.pdf
Restricted to Registered users only until 21 January 2023.

Download (519kB) | Request a copy
[img] Text (BAB V HASIL PENELITIAN)
8. BAB V HASIL PENELITIAN.pdf
Restricted to Registered users only until 21 January 2023.

Download (559kB) | Request a copy
[img] Text (BAB VI PEMBAHASAN)
9. BAB VI PEMBAHASAN.pdf
Restricted to Registered users only until 21 January 2023.

Download (272kB) | Request a copy
[img] Text (BAB VII KESIMPULAN DAN SARAN)
10. BAB VII KESIMPULAN DAN SARAN.pdf
Restricted to Registered users only until 21 January 2023.

Download (245kB) | Request a copy
[img] Text (DAFTAR PUSTAKA)
11. DAFTAR PUSTAKA.pdf

Download (271kB)
[img] Text (LAMPIRAN)
12. LAMPIRAN.pdf
Restricted to Registered users only until 21 January 2023.

Download (1MB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

This research produced ovalbumin-alginate microspheres using alginate and crosslinker BaCl2. The aim of this research was to investigate effect of concentration of alginate and BaCl2 on physical characteristics (protein loading, entrapment efficiency and yield). Microspheres were prepared by ionotropic gelation method aerosolization technique. The ratio of alginate and ovalbumin was 1:1. Concentration of alginate were 2,5 % and 3,5%, whereas BaCl2 concentration were 0,5 M and 0,75 M. The integrity of microspheres were also studied using SDS-PAGE. Results showed that ovalbumin-alginate miscrospheres had spherical and smooth particles with diameter decreased by increasing concentration of BaCl2. The size were F1 = 6,94 μm; F2 = 5,23 μm; F3 = 4,61 μm and F4 = 4,04 μm respectively. Protein loading were F1 = 66,86 % ± 10,36; F2 = 65,17 % ± 12,24; F3 = 61,34 % ± 4,16 and F4 = 53,59 % ± 2,70 while entrapment efficiency of F1 = 80,47 % ± 9,52; F2 = 81,81 % ± 10,77; F3 = 90,88 % ± 7,37 and F4 = 92,17 % ± 5,57. Yield of microspheres F1 = 60,63 % ± 3,06; F2 = 63,43 % ± 4,98; F3 = 62,93 % ± 5,96 and F4 = 73,82 % ± 10,57. Based on statistical analysis it means there were no significant differences in entrapment efficiency, protein loading and yield of all formulas. SDS-PAGE demonstrated that integrity of ovalbumin in the alginate microspheres was highly maintained by showing molecular weight of ovalbumin at 45 kDa. It can be concluded that by increasing polymer concentration and increasing crosslinking agent concentration, particle size decrease and there were no significant differences for ovalbumin content in microspheres, entrapment efficiency and yield of microspheres.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF F.07/14 Ira p
Uncontrolled Keywords: Ovalbumin, Alginate, Bacl2, Microspheres, Ionotropic Gelation, Aerosolization
Subjects: R Medicine
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
Mukhlis Bayu Irawan, -NIM051011177
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorDewi Melani, -NIDN0026027801
ContributorEsti Hendradi, -NIDN0014115703
Depositing User: Nn Deby Felnia
Date Deposited: 02 Feb 2015 12:00
Last Modified: 21 Jan 2020 10:00
URI: http://repository.unair.ac.id/id/eprint/10244
Sosial Share:

Actions (login required)

View Item View Item