Astina Yulia Subagio (2020) Deteksi Penularan Transmammary Larva Stadium 3 (L3) Pada Hospes Paratenik (Anak Mencit) Dari Induk Yang Diinfeksi Stadium Infektif (L2)Toxocara vitulorum. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Toxocariasis, as that is most often overlooked, a zoonosis, is caused by Toxocara vitulorum. Paratenic hosts are commonly used for research on the transmission of toxocariasis. Infection in the definitive host is primarily via transmammary transmission. This research evaluates the possibility of the third-stage larva of Toxocara vitulorum in various organs such as somatic muscles and viscera organs such as lungs, heart, liver, kidneys, spleen, and stomach of mice’s child after 1,2,3 weeks of breastfeeding from mother who infected by the infective larvae (L2) of Toxocara vitulorum. Mice are considered as common experimental animals. These results indicate that mice are an experimental paratenic host suitable for research on detecting the third-stage larva (L3) of Toxocara vitulorum in mice. Mice were inoculated orally with 1.000 embryonic eggs of the second stage (infective larvae) of Toxocara vitulorum that had been reared for one month with phosphate buffer saline and NaCl 0,9%. Then the female mice were mated and pregnant. After giving birth, the offspring of these mice were allowed to breastfeed according to the time group, namely 1,2 and 3 weeks after operated on. Larvae were observed microscopically, using 1 % trypsin to obtain the three-stage larva (L3). The results showed that three-stage larva via transmammary was not found on the paratenic host (mice offspring).

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