Tetri Regilya Fatimah (2020) Analisis Homologi Dan Karakteristik Filogenetik Gen Pengkode Protein Fusion (F) Regio 1363 - 2130 Bp Virus Newcastle Disease Pada Isolat Burung Dara (Columba livia domestica). Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Newcastle disease (ND) is an infectious disease caused by Avian Paramyxovirus serotype I. NDV is an RNA virus which is the major problem in Indonesianpoultry. Although vaccination program has been executed in Indonesia, Newcastle disease is still infecting pigeon and other fowl. The lack of vaccine protection against the disease, presumably due to genetic differences between vaccine strain and virus strain in the field. This research was conducted to analyze homology and phylogenetic of two out of twentieth samples isolated from domestic pigeons (Columba livia domestica) in Bratang Traditional Market are positive infected by ND. Analyze based on fusion (F) protein- encoding gene, with compared to vaccine strain and other selected isolates in GenBank. The partial F genes producing 768bp of sample was amplified using One Step RT-PCR the visualized using agarose gel electrophoresis. PCR products were purified then the sequence was run using ClustalW Alignment BioEdit ver.7. Homology scores were analyzed using Needle-Wunsch Global Align Nucleotide Sequences in NCBI. The identity score homology BD1/NDV/2019 and BD3/NDV/2019: 98% indicates possible viral similarities. BD1/NDV/2019 and BD3/NDV/2019 compared to selected isolates in GenBank, 70-80% compared to LaSota strain. Sampel was has a mutation and the cleavage site unsimilar to the various strains. The phylogenetic tree showed it has genetically close with Chicken/NDV/Banjarmasin/. This finding encouraged to develop ND vaccine based on Indonesian isolates.
| Item Type: | Thesis (Skripsi) | |||||||||
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| Additional Information: | KKC KK KH 29-21 Fat a | |||||||||
| Uncontrolled Keywords: | Fusion, Homology, ND, Phylogenetic, Pigeon. | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology |
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| Divisions: | 06. Fakultas Kedokteran Hewan > Ilmu Kedokteran Hewan Dasar | |||||||||
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| Depositing User: | prasetyo adi nugroho | |||||||||
| Date Deposited: | 08 Mar 2021 07:13 | |||||||||
| Last Modified: | 08 Mar 2021 07:13 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/104695 | |||||||||
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