Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

Suherni Susilowati and Trilas Sardjito and Imam Mustofa and Oky Setio Widodo and Rochmah Kurnijasanti (2021) Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. Animal Bioscience, 34 (2). pp. 198-204. ISSN 2765-0189, eISSN 2765-0235

[img] Text (Journal)
Bukti C 03 Effect of Green tea extract.pdf

Download (2MB)
[img] Text (Peer Review)
peer_review 3_Effect of Green Tea Extract in Extender of Simmental bull Semen on Pragnancy rate of Recipients..pdf

Download (657kB)
[img] Text (Turn It In)
Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients.pdf

Download (2MB)
Official URL: https://www.animbiosci.org/journal/view.php?number...

Abstract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination. Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12). Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows. Keywords: Artificial Insemination; Post-thawed Semen Quality; Progesterone; Transrectal Palpation

Item Type: Article
Uncontrolled Keywords: Artificial Insemination; Post-thawed Semen Quality; Progesterone; Transrectal Palpation
Subjects: S Agriculture > SF Animal culture > SF191-275 Cattle
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan > Ilmu Kedokteran Hewan Dasar
Artikel Ilmiah > SCOPUS INDEXED JOURNAL
Creators:
CreatorsNIM
Suherni SusilowatiNIDN0026065905
Trilas SardjitoNIDN0030055502
Imam MustofaNIDN0027046003
Oky Setio WidodoUNSPECIFIED
Rochmah KurnijasantiNIDN0019077004
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 15 Mar 2021 14:44
Last Modified: 15 Mar 2021 14:44
URI: http://repository.unair.ac.id/id/eprint/104841
Sosial Share:

Actions (login required)

View Item View Item