Muhammad Noor Diansyah and Ami Ashariati Prayoga and Made Putra Sedana and Merlyna Savitri and Pradana Zaky Romadhon and Putu Niken Ayu Amrita and Andi Yasmin Wijaya and Winona May Hendrata and Siprianus Ugroseno Yudho Bintoro (2019) Early detection breast cancer: role of circulating plasma miRNA-21 expression as a potential screening biomarker. Turkish Journal of Medical Sciences, 51. pp. 562-569. ISSN 1300-0144

	Text (Artikel) 5. Early detection breast cancer role of circulating plasma miRNA-21 expression as a.pdf Download (261kB)
	Text (Peer Review) Early Detection.pdf Download (1MB)
	Text (Similarity) Early detection breast cancer role of circulating plasma miRNA-21 expression as a potential screening biomarker.pdf Download (2MB)

Abstract

Background/aim: To explore the potential of the circulating plasma miRNA-21 as an early detection biomarker by comparing earlystage breast cancer (BG) and healthy control (HG) in Indonesian population. Materials and methods: The enlisted patients were 26 adult female early-stage breast cancer patients (stage 1A, 1B, 2A, and 2B) of Airlangga University Hospital from August 2019 to October 2019. Sixteen volunteers were recruited as matching healthy subjects. MiRNA-21 expression was quantified by plasma qRT-PCR. Data analysis performed using IBM SPSS Statistics v.24 (IBM Corp., Armonk, NY, USA). MiRNA-21 cut-off, sensitivity, and specificity were analyzed using receiver operating characteristic (ROC) curve. Results: The study included 26 BG and 16 HG subjects. The miRNA-21 expression in BG group was 3.933 (1.181-11.794) and 0.905 (0.164-4.532) in HG group (4.34 folds; P = 0.001), with 1.66 cut-off (92.3% sensitivity; 81.2% specificity). MiRNA-21 expression separated analysis in HG showed a 0.578 times lower expression in menopause subjects [0.651 (0.164-0.414)], compared to premenopause ones [(1.123 (0.758 - 4.532); P = 0.031]. Yet, in BG group, 1.729 times higher miRNA-21 expression was observed in menopause subjects (6.021 ± 3.583), compared to premenopause ones (3.500 ± 1.517; P = 0.022). Conclusion: Circulating miRNA-21 expression is a potential biomarker for early detection of breast cancer and might act as a breast cancer risk predictor.

Actions (login required)

View Item