Budi Utomo and Erma Safitri (2021) DNA Sequence Analysis of Follicle-Stimulating Hormone (FSH) Gene and Follicle- Stimulating Hormone Receptor (FSHR) Gene in Madrasin Cattle with Ovarian Hypofunction. Journal of Hunan University Natural Sciences, 48 (4). pp. 17-22. ISSN 1674-2974

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Abstract

The research aims to determine the profile of the follicle-stimulating hormone (FSH) gene and follicle-stimulating hormone receptor (FSHR) gene polymorphism in Madrasin cattle with ovarian hypofunction. The method applied was carried out using laboratory techniques according to PCR and DNA sequencing procedures. The samples used were 14 Madrasin cattle with ovarian hypofunction. The results of PCR of the FSH gene were shown by the presence of a band with a size of 313 bp and an FSHR gene with a band size of 306 bp. The analysis of the results for the sequence of the PCR products was carried out by comparing the nucleotide sequence of the sample with the reference nucleotide sequence. The reference nucleotide sequence is analyzed with the BioEdit ver.8 software to align the nucleotides sample and check the nucleotides' control. One sample of Madrasin cattle FSH gene PCR products and one sample of PCR control products were best selected from 14 samples that are best sequenced according to the selection after purification of DNA samples by First Base Malaysia. The samples were sequenced then BLAST to see the nucleotide sequence from the results with the available database on the website www.ncbi.nlm.nih.gov to find the similarity between a nucleotide or protein sequence (query sequence) with a database sequence (subject sequence). The sequence alignment was carried out using the BioEdit ver 8 Program. The BLAST results showed that the nucleotide sequence of FSH sequencing with the NCBI database was 94% homologous with the Bos Indicus cross Bos taurus Follicle Stimulating Hormone Beta- Subunit Gene (XM_027562775.1) and 98% homologous with the Bos Indicus cross Bos taurus Follicle Stimulating Hormone Receptor (FSHR) (XM_027555230.1). The FSH gene experience a transition mutation at base positions 16 and 21, and transversion mutations at base position 19, while the FSHR gene experience a transition mutation at bases 168 and 169. Keywords: follicle-stimulating hormone, follicle-stimulating hormone receptor, sequencing, homolog

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