PENENTUAN KONSENTRASI HAMBAT MINIMAL LARUTAN IRIGASI CHLORHEXIDINE TERHADAP BIOFILM BAKTERI Enterococcus faecalis

IBNU RUSDIARTO, 020911092 (2013) PENENTUAN KONSENTRASI HAMBAT MINIMAL LARUTAN IRIGASI CHLORHEXIDINE TERHADAP BIOFILM BAKTERI Enterococcus faecalis. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Root canal treatment is a procedure that performed to eliminate microorganisms in the root canal of the tooth with necrotic pulp. Microorganisms in a biofilm on the root canal led to the failure of root canal treatment. One of microorganisms that were able to form biofilms and cause failure of root canal treatment were the Enterococcus faecalis bacteria. The use of antimicrobial agents as a root canal irrigation were needed to eliminate microorganisms in the biofilms form. Chlorhexidine were an antimicrobial agent that is often used for root canal irrigation solution, cationic molecules of chlorhexidine would bind with a layer of extracellular polymeric matrix of biofilms that can inhibit the growth of bacteria in the biofilm. Purpose. The aim of this study is to know the minimum inhibitory concentration of chlorhexidine irrigation solution toward biofilm of Enterococcus faecalis. Method. This research was experimental laboratories in vitro research. The concentration of chlorhexidine solution were 2.5%; 1.25%, 0.625%; 0.3125%; 0.15625%. Biofilm formation was observed using the microtiter plate method, then continued by reading of Optical Density (OD) of biofilms using ELISA reader at a wavelength of 570 nm. Result. The percentage of biofilm OD exposed by concentration 2.5%; 1.25%, 0.625%; 0.3125%; 0.15625% of chlorhexidine solution in sequence were 6.59%; 8.98%; 11.24%; 13.07%; 15.17% from the positive control. Conclusion. Concentration 1.25% of chlorhexidine solution were the minimum inhibitory concentration of chlorhexidine irrigation solution toward biofilm of Enterococcus faecalis bacteria in vitro.

Item Type: Thesis (Skripsi)
Additional Information: KKA KK KG.59/13 Rus p
Uncontrolled Keywords: CHLORHEXIDINE
Subjects: R Medicine > RK Dentistry > RK1-715 Dentistry
Divisions: 02. Fakultas Kedokteran Gigi
Creators:
CreatorsEmail
IBNU RUSDIARTO, 020911092UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorKarlina Samadi, drg., MS., SpKG (K)UNSPECIFIED
ContributorDian Agustin W., Dr. drg, SpKGUNSPECIFIED
Depositing User: Mrs Nadia Tsaurah
Date Deposited: 18 Mar 2013 12:00
Last Modified: 22 Jul 2016 03:25
URI: http://repository.unair.ac.id/id/eprint/19048
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