JENNIFER WIDJAJA, 021111062 (2014) UJI TOKSISITAS XANTHONE KULIT MANGGIS (GARCINIA MANGOSTANA LINN.) TERHADAP KULTUR SEL FIBROBLAS BHK-21 (Penelitian Eksperimental Labotatoris). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background : Xanthones, from the pericarp of mangosteen (Garcinia mangostana linn., GML), are known to possess a wide spectrum of pharmacologic properties, including antioxidant, antitumor, anti-allergic, anti-inflammatory, anti-bacterial, anti-fungal, and anti-viral activities. In order to be applied as an alternative antimicrobial agents in the conservative treatment, the authors are interested in performing toxicity tests on the fibroblast cell culture BHK - 21 (Baby Hamster Kidney - 21). Objective : To examine the toxicity effect of xanthones from pericarp extract in certain concentration against fibroblast BHK-21 cell culture. Methods : This study was designed as post test only control group laboratory experiment. GML was extracted using maceration method with ethanol 96%. That extract was serial diluted from 3,13%, to 100% concentration. Toxicity was observed after 24 hours using MTT assay technique. Viable cells were measured by optical density of their MTT absorbency, and observed by ELISA reader on 620 nm. Result : Percentage of viable BHK-21 fibroblast cell culture exposed to 3,13%, 6,25%, 12,5%, 25%, 50%, and 100% concentration were 64.63%, 65.85%, 48.78%, 40.24%, 124.39%, and 132.92% respectively. Conclusion : Xanthones of GML from 3,13% to 6,25% show non-toxic activity against BHK-21 fibroblast cell culture

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