UJI SITOTOKSISITAS EKSTRAK AKAR KECUBUNG (Datura metel L.) PADA SEL FIBROBLAS DENGAN MENGGUNAKAN ESEI MTT

FITRI DWI AGUS PRATIWI, 021111373 (2015) UJI SITOTOKSISITAS EKSTRAK AKAR KECUBUNG (Datura metel L.) PADA SEL FIBROBLAS DENGAN MENGGUNAKAN ESEI MTT. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Antibiotic resistence could occur due to excessive use of antibiotics or using antibiotics without supervision of a doctor. Resistance to certain antibiotics may cause some failure in treatment of infectious disease. Root of amethyst contains antibacterial contents named flavonoid, tannin and saponin. Natural ingredients do not cause toxic effects, but scientific studies have not been performed to prove that the natural material is not toxic. Purpose. The aim of this study is to determine the toxicological properties of amethyst root extract on fibroblast BHK-21. Method. Microplate containing fibroblasts BHK-21 cells that had been exposed to three concentration of amethyst root extract (10%,17,5% and 25%) was incubated 5% CO2 at a temperature of 37º C in the incubator for 20 hours. The results of MTT assay can be seen from the absorbent solution formazan crystals through specific wavelength 620 nm with elisa reader. Result. Number of fibroblasts BHK-21 which alives after the exposure of amethyst root extract on each three concentrations shows that the viability is above 50%. Conclusion. Amethyst root extract (Datura metel L.) is not toxic against fibroblasts.

Item Type: Thesis (Skripsi)
Additional Information: KKA KK KG. 47/15 Pra u
Uncontrolled Keywords: FIBROBLAST CELL; DATURA MATEL L.
Subjects: R Medicine > RK Dentistry > RK1-715 Dentistry
Divisions: 02. Fakultas Kedokteran Gigi
Creators:
CreatorsNIM/NIDN
FITRI DWI AGUS PRATIWI, 021111373UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorTuti Kusumaningsih, Dr. drg., M.Kes.UNSPECIFIED
Depositing User: Ani Sistarina
Date Deposited: 05 Feb 2015 12:00
Last Modified: 01 Sep 2016 10:29
URI: http://repository.unair.ac.id/id/eprint/19833
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