POTENSI EKSTRAK METANOL DAUN JENGKOL (Pithecellobium jiringa) DALAM MENGHAMBAT PEMBENTUKAN BIOFILM Candida albicans

Nizamiar Hanmi, 021011032 (2015) POTENSI EKSTRAK METANOL DAUN JENGKOL (Pithecellobium jiringa) DALAM MENGHAMBAT PEMBENTUKAN BIOFILM Candida albicans. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Official URL: http://lib.unair.ac.id

Abstract

Background: Candida albicans is a dimorphic fungus in oral cavity as normal flora and can be pathogenic. Candida albicans have ability to grow into biofilm, which have a thick layer of outer skin structure called the extracellular matrix . Jengkol (Pithecellobium jiringa) contain alkaloids , flavonoids , terpenoids , and lectins which have ability as antifungal agent. Purpose: The purpose of this research is to analyze optimum dose of jengkol leaves extract using dose 100 mg/ml , 200 mg/ml , and 400 mg/ml as antibiofilm against Candida albicans biofilm. Method: Stock of Candida albicans cultured on YPD media in a 96 well microtiter plate flat bottom. There are one control group (without treatment) and the 3 treatment groups. The first treatment group jengkol leaves extract dose is 100 mg ml, the second dose is 200 mg ml, and the third dose is 400 mg/ml. Semi quantitative determination Candida albicans biofilm is done by using Crystal Violet staining method, then calculated the absorbance of the cell using a spectrophotometer with a wavelength of 570 nm. Results: The mean value of optical density control group is 1.23, a dose of 100 mg/ml is 0.2, a dose of 200 mg/ml is 0.2, and a dose of 400 mg ml is 0.21. There are significant differences between the control group and all treatment groups (P<0.05), but did not show significant differences between treatment groups (p>0.05). Conclusion: The jengkol leaves extract dose of 100 mg/ml is the optimum dose as antibiofilm against Candida albicans biofilm.

Item Type: Thesis (Skripsi)
Additional Information: KKA KK KG. 154/15 Han p
Uncontrolled Keywords: PLANT EXTRACTS; BIOFILMS
Subjects: R Medicine > RK Dentistry > RK1-715 Dentistry
Divisions: 02. Fakultas Kedokteran Gigi
Creators:
CreatorsNIM/NIDN
Nizamiar Hanmi, 021011032UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorMuhammad Luthfi, Dr drg., M.Kes.UNSPECIFIED
Depositing User: Ani Sistarina
Date Deposited: 07 Jul 2015 12:00
Last Modified: 05 Sep 2016 07:07
URI: http://repository.unair.ac.id/id/eprint/19856
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