RIESKA NURSITA, 060911009 (2013) DETEKSI AWAL KEBERADAAN Toxoplasma gondii PADA MATA MENCIT (Mus musculus) DENGAN TEKNIK POLYMERASE CHAIN REACTION (PCR). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of the study to examined the early time of Toxoplasma gondii reach the eyes post infection. Surface Antigen 1 (SAG-1) was known as the most important antigen in T. gondii tachyzoite invasion. In this study, we used SAG-1 as target genes to detect early invasion of T. gondii in the eyes by Polymerase Chain Reaction (PCR). Samples were collected every 12 hours from 18 male mices Balb/C strain which inoculated by the intraperitoneal route with 1 x 103 tachyzoites of RH strain of T. gondii. DNA extracted from whole part of eyes and amplified by PCR. The result was confirmed by agarose electrophoresis gel. Gen band with length 806 bp was found only in sample with 9 days post infection. Therefore, this result show that dissemination of T. gondii to the eyes was early detected by PCR within 9 days following intraperitoneally infection.

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