PENGARUH BERBAGAI KONSENTRASI KRIOPROTEKTAN PROPANEDIOL PADA PROSES VITRIFIKASI TERHADAP VIABILITAS EMBRIO MENCIT PASCA THAWING

AGMI WAN RATRI, 060710349 (2011) PENGARUH BERBAGAI KONSENTRASI KRIOPROTEKTAN PROPANEDIOL PADA PROSES VITRIFIKASI TERHADAP VIABILITAS EMBRIO MENCIT PASCA THAWING. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This experiment was doing to know the effects concentration of cryoprotectant at vitrification on viability of mice’s embryos. Embryos were exposed to a different concentration of propanediol cryoprotectant, that is concentration 10%, 20% and 30%. Embryos were kept in mini straw and then plunged into liquid nitrogen rapidly. After 2 week, the frozen embryos were thawed in a 30°C water bath and be washed twice in 0,5 M Sucrose. Viability of embryos was examined used inverted microscope. The result showed there have a significant differences (p < 0,05) for embryos survival among treatment of propanediol 10% (46,95 %), propanediol 20% (51,73 %) and propanediol 30% (54,94 %). A various concentration of cryoprotectan was give a different result at embryos viability. The conclusion of this experiment there is a real difference (p < 0,05) at a various concentration of propanediol cryoprotectant with a good result give in propanediol 30% (54,94 %).

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 70 11 Rat p
Uncontrolled Keywords: vitrification, cryoprotectant, concentration, viability, embryos
Subjects: Q Science > QL Zoology > QL700-739.8 Mammals
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
S Agriculture > SF Animal culture > SF405.5-407 Laboratory animals
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsEmail
AGMI WAN RATRI, 060710349UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorWidjiati, Dr., drh., M.SiUNSPECIFIED
ContributorM. Zainal Arifin, Dr., drh., MSUNSPECIFIED
Depositing User: Nn Dhani Karolyn Putri
Date Deposited: 16 Dec 2011 12:00
Last Modified: 30 Sep 2016 01:36
URI: http://repository.unair.ac.id/id/eprint/20863
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