UJI SPESIFISITAS EPIDERMAL GROWTH FACTOR (EGF) PADA OOSIT SAPI YANG DIMATURASI SECARA IN VITRO DENGAN METODE DOT BLOTTIN

AULIA REZA PRADIPTA, 060710019 (2011) UJI SPESIFISITAS EPIDERMAL GROWTH FACTOR (EGF) PADA OOSIT SAPI YANG DIMATURASI SECARA IN VITRO DENGAN METODE DOT BLOTTIN. Skripsi thesis, UNIVERSITAS AIRLANGGA.

[img]
Preview
Text (ABSTRAK)
gdlhub-gdl-s1-2012-pradiptaau-18334-kh1331-u.pdf

Download (98kB) | Preview
[img] Text (FULLTEXT)
gdlhub-gdl-s1-2012-pradiptaau-18334-kh1331-u.pdf
Restricted to Registered users only

Download (994kB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

The aim of this study was to know the character of epidermal growth factor (EGF) protein isolated from bovine oocyte. Bovine ovary was collected from slaughter house washed with NaCl. An aspiration technique was used to collect oocyte. Those oocytes were maturated by in vitro condition then the EGF protein was isolated after running in SDS PAGE. Polyclonal antibody was produced by sub cutaneous injected of crude protein oocytes containing EGF in rabbit, then followed by dot blotting examination. Booster was done twice after 3rd and 7th bleeding then continued until 10 times every week. The antibody anti EGF from rabbits sera was reacted with EGF antigen. The dot blot showed, the intensity of dot color increased significantly from 4th bleeding and reaches the highest concentration of antibody at 9th bleeding. It meant, bovine oocyte EGF protein could be characterized by dot blotting, of which the highest antibody titer detected at eleventh week after sub cutaneous injection of crude protein oocytes containing EGF.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 133 11 Pra u
Uncontrolled Keywords: epidermal growth factor, in vitro maturation
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
AULIA REZA PRADIPTA, 060710019UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorDady Soegianto Nazar, Dr. , MSc., drhUNSPECIFIED
Depositing User: Dwi Marina
Date Deposited: 16 Jan 2012 12:00
Last Modified: 08 Aug 2016 07:19
URI: http://repository.unair.ac.id/id/eprint/20924
Sosial Share:

Actions (login required)

View Item View Item