PENGARUH BERBAGAI KONSENTRASI ETILEN GLIKOL PADA VITRIFIKASI TERHADAP VIABILITAS EMBRIO MENCIT PASCA THAWING

VIVIYANTI UDAYANI INA OSE, 060710240 (2011) PENGARUH BERBAGAI KONSENTRASI ETILEN GLIKOL PADA VITRIFIKASI TERHADAP VIABILITAS EMBRIO MENCIT PASCA THAWING. Skripsi thesis, UNIVERSITAS AIRLANGGA.

[img]
Preview
Text (ABSTRAK)
gdlhub-gdl-s1-2011-oseviviyan-22035-kh1471-k.pdf

Download (333kB) | Preview
[img] Text (FULLTEXT)
gdlhub-gdl-s1-2012-oseviviyan-18370-kh1471-p.pdf
Restricted to Registered users only

Download (1MB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

The aim of this research was to investigate the effect of various concentrations using ethylene glycol (EG) on vitrification to viability of mice embryo post thawing. The collected embryos were washed using PBS and divided in three groups which are EG 10%, 20% and 30%. Embryos were exposed inside cryoprotectant at 200C for 5 minutes. It were putted inside the straw than added by 0.5 M sucrose. Straws were exposed 10 second, then directly inside liquid nitrogen. Frozen embryos were thawed in waterbath at 370C for 30 seconds. It were washed sequently by 0.5 M and 0.1 M of sucrose to removed cryoprotectant. Inverted microscope were used to examine the viability of mice embryo. The result showed that there was significantly different (p < 0.05) between various concentration using EG. Rate of viability were 69.22+6.88, 90.00+8.94, 77.77+9.93. Therefore, there was effect from various concentration EG on vitrivication to viability of mice embryo post thawing, with the highest percentages of viability were 20%.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 147 11 Ose p
Uncontrolled Keywords: COW - GEMPLASLSM RESOURCES - CRYOPRESERVATION
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
VIVIYANTI UDAYANI INA OSE, 060710240UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorNanik Sianita W, , S.U., drh.UNSPECIFIED
Depositing User: Dwi Marina
Date Deposited: 18 Jan 2012 12:00
Last Modified: 08 Aug 2016 09:32
URI: http://repository.unair.ac.id/id/eprint/20928
Sosial Share:

Actions (login required)

View Item View Item