DAYA TAHAN HIDUP SEL MONOLAYER KUMULUS OOSIT SAPI MADURA BERDASAR KONSENTRASI HORMON ESTROGEN YANG DIHASILKAN

UTAMININGSIH, FAJAR ISTIKOMAH, NIM. 060313191 (2009) DAYA TAHAN HIDUP SEL MONOLAYER KUMULUS OOSIT SAPI MADURA BERDASAR KONSENTRASI HORMON ESTROGEN YANG DIHASILKAN. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The research is about Madurese cow monolayer cumulus oocyte cell culture. The aim of this research was to know that monolayer cumulus oocyte cell can growth on the third, sixth, ninth, and twelfth day incubation time period during cultured, so that we can know maximum and minimum potency life time of monolayer cumulus oocyte cell based on its produce of estrogen hormone concentration. The cell breeding was made from aspiration of Madurese cow ovarium cumulus cell then bred in TCM 199 with the addition of FCS and gentamicyn 5 µl/ ml in the temperature 38°C, pressure 5% CO2, and covered with mineral oil. Cell product harvested after three, six, nine, and twelve day of incubation. The highest estrogen concentration (lll,lla ± 27,01) from cumulus cell breeding produced after six day incubation and the lowest estrogen concentration (36,00b ± 10,11) produced after twelve day incubation. There was significantly difference (p< 0,01) estrogen concentration which is produced by Madurese cow monolayer cumulus oocyte after three, six, nine, and twelve day incubation.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 69 08 Uta d
Uncontrolled Keywords: OOCYTES; ESTROGEN
Subjects: S Agriculture > SF Animal culture > SF191-275 Cattle
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsEmail
UTAMININGSIH, FAJAR ISTIKOMAH, NIM. 060313191UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorLaba Mahaputra, Prof. Dr., M. Sc., Drh.UNSPECIFIED
Depositing User: Sulistiorini
Date Deposited: 04 Jun 2009 12:00
Last Modified: 31 Aug 2016 04:32
URI: http://repository.unair.ac.id/id/eprint/21180
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