IDENTIFIKASI Growth Differentiation Factor-9 (GDF-9) PADA OOSIT YANG DIKOLEKSI DARI FOLIKEL PREANTRAL SAPI SETELAH DIMATURASI In Vitro

DESI WULANSARI, 060610153 (2011) IDENTIFIKASI Growth Differentiation Factor-9 (GDF-9) PADA OOSIT YANG DIKOLEKSI DARI FOLIKEL PREANTRAL SAPI SETELAH DIMATURASI In Vitro. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Growth Differentiation Factor-9 (GDF-9) is a glycoprotein belonging to the subfamily of the Transforming Growth Factor Beta (TGFβ) secreted by the oocyte with a molecular weight of 51 kDa. GDF-9 stimulating granulosa cells proliferation and inhibiting differentiation. The purpose of this study is to indentify GDF-9 in oocytes collected from preanthral bovine follicle which matured in vitro. This study uses a method based on the specificity immunocytochemistry bond between avidin and biotin molecules contained in the secondary antibody avidin-biotin complex shape. Sample, bovine ovaries, are collected from RPH Penggirian Surabaya. This study using 105 matured oocytes from 62 ovaries. Immunocytochemistry result showed 77,14% GDF-9 identified. It means GDF-9 playing a rule in maturating oocytes which collected from preanthral follicle.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 09/11 Wul i
Uncontrolled Keywords: PROTEIN IN ANIMAL NUTRITION; CATTLE - EMBRYOS - TRANSPLANTATION
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
DESI WULANSARI, 060610153UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorWurlina Meles, Prof. Dr., M.S., drh.UNSPECIFIED
Depositing User: mat sjafi'i
Date Deposited: 21 Jul 2011 12:00
Last Modified: 13 Sep 2016 02:00
URI: http://repository.unair.ac.id/id/eprint/21204
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