THERESIA AUDITA GURETTI, 060610131 (2011) UJI SPESIFISITAS PROTEIN KIT LIGAND YANG DIISOLASI DARI OOSIT SAPI MATURASI DENGAN METODE DOT BLOTTING. Skripsi thesis, UNIVERSITAS AIRLANGGA.

Preview	Text (ABSTRAK) gdlhub-gdl-s1-2011-gurettithe-19247-kkckkk-k.pdf Download (13kB) | Preview
	Text (FULL TEXT) gdlhub-gdl-s1-2011-gurettithe-16079-kkckkk-u.pdf Restricted to Registered users only Download (950kB) | Request a copy

Abstract

Embryo Transfer was one of reproduction technology used to increase cattle productivity. The succeed of embryo transfer depends on the quality of the embryo. The Quality of oocyte for in vitro fertilization in order to produce in vitro embryo was influenced by the in vitro maturation process. To reveal the maximum competence of the oocyte was also influenced by some factors produced by the primordial follicle which has the function to initiate growth into primary follicle. One of those factors was Kit-Ligand. Kit Ligand (KL) was a growth factor bind to C-Kit receptor which was actively involved in initiating the beginning of primordial follicle growth process in the ovary. The lack presence of Kit Ligand could cause malfunction in the oocyte growth. This research consisted of collecting oocyte and in vitro culturing, protein isolation, electro elution to purified Kit-Ligand isolate, producing polyclonal antibody, immunization on experimental animal, and Specification test of Kit Ligand using dot blotting method. The presence of color changing showed specification between the protein as antigen and the polyclonal antibody. Dot blotting method could also confirm the concentration of antibody based on the time difference on collecting sample. The result showed on the second week the immune respond increased. It increased gradually until the fourth week (second bleeding) and then decreased. The production of antibody began to increase again and reach the top on the eighth week (sixth bleeding).

Actions (login required)

View Item