ALMAEDAWATI ERINA, 060513435 (2011) UJI AKTIVITAS ANTIBAKTERI Streptomyces sp.-1 DAN Streptomyces sp.-2 ISOLAT TANAH EKOSISTEM MANGROVE PANTAI TIMUR SURABAYA TERHADAP Mycobacterium tuberculosis. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Antibacterial activity test of Streptomyces sp.-1 and Streptomyces sp.-2 isolated from East coast of Surabaya mangrove ecosystems soil against Mycobacterium tuberculosis H37Rv. This research has done in vitro using diffusion modifications Agar plates method to know antibiotic production of Streptomyces sp. Against M.tuberculosis H37Rv by the formation of diameter inhibition zone around the colonies of M.tuberculosis H37Rv. The activity test has done by sticking a printout of Agar plates inoculated by Streptomyces sp. on the media that has been inoculated by M.tuberculosis H37Rv, then incubated at 37°C for 3-5 weeks. Positive results are indicated by clear zone produced by Streptomyces sp. around M.tuberculosis H37Rv colonies on the petri dish it means that the isolates of Streptomyces sp. can produce antibiotic to inhibit the growth of M.tuberculosis H37Rv. Results showed that Streptomyces sp.-1 isolated from East coast of Surabaya mangrove ecosystems soil is potensial in producing antibiotic because it showed a result inhibiting the grown of M.tuberculosis H37Rv that marked with no growth of M.tuberculosis H37Rv on Petri dish. Streptomyces sp.-2 isolated from East coast of Surabaya mangrove ecosystems soil can inhibit the growth of M.tuberculosis H37Rv that marked with the formation of inhibition zone around M.tuberculosis H37Rv colonies on petri dish cultures of 18,9 mm.

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