IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) PADA OOSIT SAPI YANG DIKOLEKSI DARI FOLIKEL BERDIAMETER 2-5 mm

Lutfiana Widya Larasanti, 060610258 (2012) IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) PADA OOSIT SAPI YANG DIKOLEKSI DARI FOLIKEL BERDIAMETER 2-5 mm. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Growth differentiation factor-9 (GDF-9) is a 51 kDA glycoprotein as a part of Transforming growth factor-beta (TGF-β) subfamily secreted by oocytes. GDF-9 plays role in oocyte maturation by stimulating granulosa cells proliferation and inhibiting differentiation. The purpose of this study was to identify GDF-9 expressed in bovine oocytes that were collected from follicle with 2-5 mm diameter. This study used a method based on specific immunoaffinity binding between avidin molecules biotinilated into the secondary antibody (avidin-biotin complex). GDF-9 was expressed in 38 oocytes out of 48 collected oocytes (79.17%). Expression was characterized by dark-brown colour due to chromogen visualization of DAB in the SA-HRP complex boundaries. GDF-9 down-regulated oocytes were methylen green counterstained

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH. 62 / 12 Lar i
Uncontrolled Keywords: PROTEIN IN ANIMAL NUTRITION; CATTLE
Subjects: Q Science > QL Zoology
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsEmail
Lutfiana Widya Larasanti, 060610258UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorSuzanita Utama, drh., M.PhilUNSPECIFIED
ContributorNgakan Made Rai Widjaja, Dr. drh., M.S.UNSPECIFIED
Depositing User: Mrs Nadia Tsaurah
Date Deposited: 15 Oct 2012 12:00
Last Modified: 28 Jul 2016 04:31
URI: http://repository.unair.ac.id/id/eprint/21490
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