IKA WAHYUNI, 061011046 (2014) DETEKSI REAKSI SILANG PROTEIN Raillietina tetragona DENGAN ANTIBODI Ascaridia galli MENGGUNAKAN UJI ELISA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The objective of this study is to find out cross reaction between antigens of Raillietina tetragona with antibody of Ascaridia galli by indirect-ELISA technique. In the first phase, collected Raillietina tetragona and Ascaridia galli from chicken’s small intestine and created homogenate by sonication and centrifuge to got homogenate. The second phase, polyclonal antibody of Raillietina tetragona and Ascaridia galli was obtained from homogenate immunizated in mice. The third phase, cross reaction was detected by indirect-ELISA. The result showed : 1) Mean optical density (OD) value of the control antibody was 0.105; serum immunizated by Raillietina tetragona was 0.237 and serum immunizated by Ascaridia galli was 0.444. 2). Statistical analysis used was Duncan’s multiple range test 1% indicated significant difference (p<0,01) between P0, P1, and P2, so the conclution based on the analysis above there was cross reaction between antigens of Raillietina tetragona with antibody of Ascaridia galli.

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