Ainun Jariyah Nofianti, 060032847 (2008) DETEKSI ANTIBODI PADA MENCIT YANG DIINFEKSI Toxocara cati TERHADAP WAKTU YANG BERBEDA MENGGUNAKAN TEKNIK INDIRECT ELISA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this attempt is to know how the pattern of humoral immune responses toward infection of Toxocara cati at different times by using indirect ELISA’s technique. The animal object that used as analisys object is five male mice (Mus musculus) which infection with second stage larvae of Toxocara cati worm, and than conducted by intake of serum at four different time to detect imunoglobulin formed from the whole animal of the attempt. T0 is serum intake before conducted by infection while T1, T2, and T3 are serum intake on seventh, fourteenth and twenty eight days after conducted by oral infection. Humoral immune responses toward second stage larva of Toxocara cati worm determined by pursuant value of optical density (OD) that obtained from assay of indirect ELISA, where value of the OD explaining the titers of antibody in serum. Afterwards, data obtained to be analysed whith Anova Test using SPSS 13.0 for windows and than to be tested by Duncan 5%. The result of research show highly significant difference (p<0,01) among T0, T1, T2, and T3. After continued with test of Duncan, clearly there are significanly difference (p<0,05) among T0 by T1, T2, and T3. While among T1, T2, and T3 there no significanly difference.

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