PRODUKSI ANTIBODI POLIKLONAL EARLY PREGNANCY FACTOR (EPF) PADA KAMBING SETELAH IMUNISASI EPF DARI KOTILEDON SAPI FRIESIAN HOLSTEIN (FH)

Sonny Handaru, 060413318 (2008) PRODUKSI ANTIBODI POLIKLONAL EARLY PREGNANCY FACTOR (EPF) PADA KAMBING SETELAH IMUNISASI EPF DARI KOTILEDON SAPI FRIESIAN HOLSTEIN (FH). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of the present study was to investigate goat EPF polyclonal antibody of induction of isolated Friesian Holstein ox cotyledon. EPF through fourteen weeks incubation in vivo production. Induction of isolated Friesian Holstein ox cotyledon EPF was applied in one males Ettawa crossbreed goat. Initially, goat were sub cutaneously injected active immunization with isolated EPF in Completed reund'S Adjuvant (CFA). Two weeks post first immunization was performed booster with isolated EPF in an Incomplete Freund'S Adjuvant (IFA). Further, the next booster was done every three week. Before booster, was always executed taking serum every week. Then, EPF polyclonal antibody of goat was measured by Elisa indirect. The results of ELISA showed increasing of goat antibody titers. The conclusion of this research proved that isolated Friesian Holstein ox otyledon EPF can induced humoral antibody response. Optimal titer value was reached in 3rd booster that is 245,142 μg/ml.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH . 99/08 Han p
Uncontrolled Keywords: EPF, ETTAWA CROSSBREED GOAT, POLYCLONAL ANTIBODY
Subjects: Q Science > QL Zoology > QL1-991 Zoology
R Medicine > RG Gynecology and obstetrics > RG1-991 Gynecology and obstetrics > RG500-991 Obstetrics > RG551-591 Pregnancy
S Agriculture > SF Animal culture > SF380-388 Goats
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsEmail
Sonny Handaru, 060413318UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorTjuk Imam Restiadi, M.Si., drhUNSPECIFIED
ContributorRahmi Sugihartuti, M.Kes., drhUNSPECIFIED
Depositing User: Nn Dhani Karolyn Putri
Date Deposited: 18 Aug 2010 12:00
Last Modified: 09 Jul 2017 16:25
URI: http://repository.unair.ac.id/id/eprint/21735
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