PENGARUH PENYUNTIKAN ISOLAT PROLAKTIN ITIK FASE MOULTING TERHADAP TIMBULNYA ANTIBODI POLIKLONAL ANTI PROLAKTIN KAMBING

Resmita Citra Rizqi, 060413360 (2008) PENGARUH PENYUNTIKAN ISOLAT PROLAKTIN ITIK FASE MOULTING TERHADAP TIMBULNYA ANTIBODI POLIKLONAL ANTI PROLAKTIN KAMBING. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

An experiment was conducted to produce anti prolactin polyclonal antibody and get the highest optical density (OD) result after injection isolate prolactin goat. Raising anti prolactin polyclonal antibody used six male local goats, five male local goats injected with 275 μg/ml isolate prolactin in Complete Freund’s Adjuvant (CFA) and one male local goat injected with PBS as a control. The first booster (repeat injection) had done with 275 μg/ml isolate prolactin in Incomplete Freund’s Adjuvant on the 12th days. One day before the first injection and every week after the first booster until the 13th booster (the last booster) did bleed two times. ELISA Indirect test was perform the appearence anti prolactin polyclonal antibody as well as the highest OD result. ANOVA test (Analysis of Variance) was apply in order to identify the difference among dose treatments then continue using Honestly Significant Difference test (5 %). These results anti prolactin polyclonal antibody was appear at first bleeding and the highest OD antibody polyclonal anti prolactin in the 17th bleeding (1,00467 ± 0,47112). The conclusion strongly indicated anti prolactin polyclonal antibody for duck could be produced from the goat with OD (1,00467 ± 0,47112).

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH . 150/08 Riz p
Uncontrolled Keywords: DUCK, GOAT, MOULTING, PROLACTIN, POLYCLONAL ANTIBODY
Subjects: Q Science > QD Chemistry > QD1-999 Chemistry
Q Science > QH Natural history > QH301 Biology
S Agriculture > SF Animal culture > SF380-388 Goats
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
Resmita Citra Rizqi, 060413360UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorMas'ud Hariadi, Prof., Drh., M.Phill., Ph.DUNSPECIFIED
ContributorSri Hidanah, Dr., Ir., M.SUNSPECIFIED
Depositing User: Nn Dhani Karolyn Putri
Date Deposited: 25 Aug 2010 12:00
Last Modified: 01 Oct 2016 03:18
URI: http://repository.unair.ac.id/id/eprint/21773
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