Rofik Fadillah, 060112944 (2006) PENGGUNAAN ANTI CASPASE 8 SEBAGAI PENGHAMBAT APOPTOSIS PADA KULTUR SEL FIBROBLAS YANG DIPAPAR VIRUS GUMBORO. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research was to evaluate whether anti caspase 8 can inhibit apoptosis in Fibroblast culture cell which infected by Infectious Bursal Disease Virus. Fibroblast cell cultured in Minimal Essential Medium (MEM) supplemented with fetal bovine serum (FBS), 100 iu/ml penicilline, 100 pg/ml streptomcine. Then culture cell infected by Infectious Bursal Disease Virus with titer 10-J then incubated in 30 minutes and added anti caspase 8 with titer 10"2 in various incubation intervals, 15, 30, 60, 90, 120 and 150 minutes. After treated, the cell was collected and washed with PBS. The DNA fragmentation of apoptosis assay with composite gel electrophoresis which colloured with silver (AgNO3). The result showed that anti caspase 8 able to inhibit apoptosis caused by Infectious Bursa( Disease Virus infection. Fragmentatian DNA of apoptosis can inhibited after incubation anti caspase 8 in 1 50 minutes.

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