HUBUNGAN ENDONUCLEASE-G DENGAN KEJADIAN APOPTOSIS SEL AMNION PADA KEHAMILAN DENGAN KETUBAN PECAH DINI

BENEDICTUS TRIAGUNG RUDDY PRABANTORO, 090315221 D (2013) HUBUNGAN ENDONUCLEASE-G DENGAN KEJADIAN APOPTOSIS SEL AMNION PADA KEHAMILAN DENGAN KETUBAN PECAH DINI. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study is to know whether there is an association between endonuclease-G and the apoptosis of amniotic cells in pregnancies with premature rupture of the membranes. Through research study on the amnion cell apoptosis mechanism in pregnancies with premature rupture of the membranes, it is possible to know whether endonuclease-G is the biomarker of amnion cells apoptosis involved. Methods: Analytic observational methods with cross-sectional study have been used in this study design. Sample tissues from the ruptured amniotic membrane of 41 pregnant women with premature rupture of the membranes were taken and processed to be analyzed further; TUNEL methods were used for obtaining the apoptosis index analysis of amnion tissue; Western-Blotting methods were used in both the analysis of endonuclease-G (30kDa/full length) expression and also the analysis of caspase-3 (35 kDa/full length) expression. Data, consisting age of pregnancies, endonuclease-G, caspase-3 and apoptosis index were analyzed using the chi-square test. The results of the study showed that apoptosis was significantly associated with the high level detection of endonuclease-G (p=0,000) but was not associated with caspase-3 significantly (p=0,316). It was also found that the apoptosis in premature rupture of the membranes in pregnancy is not dependent to the age of pregnancies. Therefore, there is an indication that the occurrence of apoptosis is dominant through the caspase-independent pathway (endonuclease-G). Hence it can be concluded from the results of this study that high level detection of endonuclease-G is a biomarker of apoptosis of amnion cells in pregnancies with premature rupture of the membranes

Item Type: Thesis (Disertasi)
Additional Information: KKA KK Dis K01/13 Pra h
Uncontrolled Keywords: Endonuclease-G, Caspase-independent, PROM/PPROM
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: 09. Sekolah Pasca Sarjana > Ilmu Kedokteran
Creators:
CreatorsNIM/NIDN
BENEDICTUS TRIAGUNG RUDDY PRABANTORO, 090315221 DUNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorR. Prajitno Prabowo, Prof., dr, SpOG.UNSPECIFIED
ContributorNi Made Mertaniasih, Prof. Dr., dr. MS, SpMKUNSPECIFIED
ContributorFedik A. Rantam, Prof. Dr., drh.UNSPECIFIED
Depositing User: mat sjafi'i
Date Deposited: 05 Sep 2016 07:10
Last Modified: 05 Sep 2016 07:10
URI: http://repository.unair.ac.id/id/eprint/32112
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