KARAKTERISASI PROFIL METABOLIT DAN UJI TOKSISITAS IN VITRO EKSTRAK DAUN KATUK (Sauropus androgynus), SEBAGAI UPAYA PENGUJIAN KEAMANAN SUPLEMEN HERBAL

OEKE YUNITA, - (2012) KARAKTERISASI PROFIL METABOLIT DAN UJI TOKSISITAS IN VITRO EKSTRAK DAUN KATUK (Sauropus androgynus), SEBAGAI UPAYA PENGUJIAN KEAMANAN SUPLEMEN HERBAL. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The important effect of Sauropus androgynus / SA (katuk), as a lactagogum for increasing human breast-milk production in Indonesia, must face the reality that there are also many investigations revealing its side effect, associated with Bronchiolitis obliterans, in Taiwan and Japan. The occurence of side effects from SA makes toxicological controlled studies necessary to assess its safety. Concerning about the complicated causes which can influence the result of toxicity assay on herbal supplements, initial step for safety assessment will begin with authentication of plant raw material by metabolic profiling of SA from several areas on East Java, Indonesia, namely Surabaya, Trenggalek, Bojonegoro, Purwodadi, Purwosari and Batu, by Gas Chromatography-Mass Spectrometry Detector (GC-MSD). The result of this study showed that GC-MSD analysis of SA samples could show different metabolic profiles among samples from different geographical areas. Statistical analysis could classify all SA samples into five clusters that each SA sample from areas with similar geographical conditions could be grouped in the same cluster, except SA sample from Purwosari (PWS). Toxicity assay of SA on mesenchymal stem cells derived from human bone marrow could show that SA methanolic crude extract could inhibit cell viability via apoptosis pathway. The metabolic profiles of SA from several areas on East Java had a good correlation with toxic effect of SA samples, while the SA compound that was highly correlated with SA toxicity is phytol.

Item Type: Thesis (Disertasi)
Additional Information: KKC KK Dis M 03/12 Yun k
Uncontrolled Keywords: Sauropus androgynus, metabolic profiling, in vitro toxicity assay. mesenchymal stem cells-derived from human bone marrow
Subjects: Q Science > QD Chemistry > QD1-999 Chemistry
Q Science > QD Chemistry > QD241-441 Organic chemistry
Q Science > QK Botany > QK1-989 Botany
Divisions: 09. Sekolah Pasca Sarjana > Ilmu Matematika & IPA
Creators:
CreatorsNIM/NIDN
OEKE YUNITA, -UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorMochammad Yuwono, Prof.Dr.rer.nat.,MSUNSPECIFIED
Depositing User: Nn Anisa Septiyo Ningtias
Date Deposited: 20 Jun 2017 16:51
Last Modified: 20 Jun 2017 16:51
URI: http://repository.unair.ac.id/id/eprint/32779
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