SENYAWA FENOLIK DARI EKSTRAK KULIT BATANG TRENGGULI (Cassia fistula) YANG AKTIF SEBAGAI ANTIOKSIDAN

HERMIEN NOORHAJATI, 090810019D (2013) SENYAWA FENOLIK DARI EKSTRAK KULIT BATANG TRENGGULI (Cassia fistula) YANG AKTIF SEBAGAI ANTIOKSIDAN. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Free radicals give negative effect to human health. They can cause many diseases such as cancer, coronary heart diseases, diabetic mellitus, arteroschlerosis, rheumatic arthritis, liver troubles, and early aging process. The free radicals need to be neutralized by antioxidant. There are plenty natural antioxidants in the plants as a phenolic compounds, flavonoid, stilbene, anthraquinone, phenylpropanoid and xanthone. This research is conducted to get the phenolic compounds from extract stem bark of Cassia fistula that has an antioxidant activity. The material of this research is the stem bark of Cassia fistula. The stem bark of the Cassia fistula is extracted with the methanol solvent, then the methanol extract is partitioned with n-heksana and ethyl acetate, and finally get the extract n-heksana, extract ethyl acetate, and extract methanol. Through these three extracts, we conducted a total phenol test, DPPH test, and Lipid Peroxide test (LPO) by using the ferry thiocyanate (FTC) method. These three tests show that extracts of the ethyl acetate have the potential as antioxidant. Separation and purification of ethyl acetate extract is conducted by using many chromatographic techniques which at the end we can get four pure compounds. The molecule structure of the four isolation result is set up based on the analysis and spectroscopic result that includes the data of spectrum ultraviolet (UV), Nucleus Magnet Resonance (NMR) one-dimension (1D), NMR-2D (Heteronuclear Multiple Quantum Coherence/HMQC and Heteronuclear Multiple Bond Connectivity/HMBC), and mass spectrum (Liquid Chromatographi Mass Spectra/LC-MS). The compounds are 4,3’ - dih ydro xy -3,5’ 3,5’ -dimet dimet dimet hoxy stilben stilben stilben e (F-1), 5,7,4’ , 5,7,4’ -tri hydro xyfllavone/ xyfllavone/ xyfllavone/ xyfllavone/ xyfllavone/ xyfllavone/ apigenin (F-2), 3,7 - dihydroxy-4’ -met hoxy flava flava n (F-3), and 7-hydro dro xy -6-met hoxy -2H-chromen chromen chromen -2-one/ one/ one/ scopoletin (F-4). The three isolated phenolic compounds F-1, F-2, and F-3 never been reported before as a chemical content in Cassia fistula plant. The result of lipid peroxide activity test through FTC method on those four compounds shows that the lipid peroxide activity compound F-2 > compound F-3 > compound F-4 > compound F-1.

Item Type: Thesis (Disertasi)
Additional Information: KKC. KK Dis. M. 08/13 Noo s
Uncontrolled Keywords: Trengguli, Cassia fistula’s stem bark, antioxidants, lipid peroxide, ferry thiocyanate.
Subjects: Q Science > QD Chemistry > QD241-441 Organic chemistry
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 09. Sekolah Pasca Sarjana > Ilmu Matematika & IPA
Creators:
CreatorsNIM/NIDN
HERMIEN NOORHAJATI, 090810019DUNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorAmi Soewandi J.S.,, Prof. Dr. Apt.UNSPECIFIED
Depositing User: Dwi Prihastuti
Date Deposited: 20 Sep 2016 04:29
Last Modified: 10 Jul 2017 23:40
URI: http://repository.unair.ac.id/id/eprint/32915
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