PERBANDINGAN WAKTU PEMBELAHAN BAKTERI ANTARA GALUR MULTIRESISTEN DAN NON-MULTIRESISTEN Methicillin Resistant Staphylococcus aureus (MRSA) ISOLAT PASIEN MALANG SECARA IN VITRO

DEWI, ERIKAWATI (2015) PERBANDINGAN WAKTU PEMBELAHAN BAKTERI ANTARA GALUR MULTIRESISTEN DAN NON-MULTIRESISTEN Methicillin Resistant Staphylococcus aureus (MRSA) ISOLAT PASIEN MALANG SECARA IN VITRO. Thesis thesis, UNIVERSITAS AIRLANGGA.

[img] Text (FULLTEXT)
gdlhub-gdl-s2-2015-erikawatid-39607-tkd03-1-f.pdf
Restricted to Registered users only

Download (2MB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

Background : One of the virulence factor of bacteria is the colonization which depend on the growth rate. Multiresistant bacteria was believed to have a slower growth rate than non-multiresistant bacteria. Aim :To investigate whether there was differences in doubling time of multiresistant and non-multiresistant strain of MRSA. Method : We obtained susceptibility profile to eight classes of antibiotics. The calculation of doubling time was performed as an overnight of bacterial cultures were diluted 1 : 100 in 5 mL of BHI medium and grown for 3 hours at 37°C, 180 rpm. These cultures were seeded at a 1:100 dilution in another 5 mL BHI medium and incubated at 37°C,180 rpm for futher 4 hours. Optical density was measured every 30 until 4 hours. Values were converted into log2 values, and the doubling time was calculated as the reciprocal of the slope. Results :The mean of doubling time of multiresistant strain of MRSA was 27.9 + 5.80 minutes, whereas non-multiresistant strain of MRSA was 22.3 + 0.99 minutes (p<0.05). In addition, the logarithmic phase was different significantly between groups (p<0.05). There was a correlation between the duration of logarithmic phase and doubling time (p<0.05). Conclusion :The doubling time of multiresistant strain of MRSA was significantly longer than the doubling time of non-multiresistant strain of MRSA.

Item Type: Thesis (Thesis)
Additional Information: KKA KK TKD. 03/15 Eri p
Uncontrolled Keywords: Multiresistant, non-multiresistant, MRSA, doubling time.s
Subjects: Q Science > QR Microbiology
Divisions: 01. Fakultas Kedokteran
Creators:
CreatorsEmail
DEWI, ERIKAWATIUNSPECIFIED
Contributors:
ContributionNameEmail
ContributorEddy Bagus Wasito, Prof. Dr. , dr., MS., SpMK(K)UNSPECIFIED
Depositing User: Nn Aimmatul Mukaromah
Date Deposited: 2016
Last Modified: 30 Oct 2016 19:58
URI: http://repository.unair.ac.id/id/eprint/33815
Sosial Share:

Actions (login required)

View Item View Item