EFEK ANTIBAKTERI EKSTRAK ETANOL BUNGA ROSELA (Hibiscus sabdariffa L.) TERHADAP PERTUMBUHAN DAN PERUBAHAN STRUKTUR DINDING SEL BAKTERI METHICILLIN-RESISTANT Staphylococcus aureus (MRSA)

Swaidatul Masluhiya AF, 011214253010 (2015) EFEK ANTIBAKTERI EKSTRAK ETANOL BUNGA ROSELA (Hibiscus sabdariffa L.) TERHADAP PERTUMBUHAN DAN PERUBAHAN STRUKTUR DINDING SEL BAKTERI METHICILLIN-RESISTANT Staphylococcus aureus (MRSA). Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This study is aimed to investigate the antibacterial effect of ethanol extract from rosella flower on MRSA in vitro, to observe the relationships between giving phased concentration of Rosella flower extract and the growth obstruction of MRSA by in vitro, and to find out the picture of structural change of MRSA cell wall in the given the extract of rosella flower in some concentration. The selected method of antibacterial test is dilution method in order that by mixing MHA medium with the extract of rosella flower. The change of the cell wall structure of MRSA bacteria is observed with SEM with 5.000 - 10.000 magnification. The obtained data are analyzed with ANOVA-95% confidence interval, pearson correlation to prove the presence of relationships giving phased concentration of rosella flower extract and the growth obstruction of MRSA, and the result of SEM observation is explained descriptively. The result of antibacterial test shows that the ethanol extract of rosella flower has antibacterial activity against MRSA with MIC at 0,25x104 μg/m and MBC at 1x104 μg/m concentration. The extract of rosella flower at control, the cell morphology of MRSA bacteria still looks intact with coccus shape, smooth and protrudes. At 0,25x104 μg/m, the cell morphology of MRSA bacteria still looks intact, in coccus shape with cell diameter relatively bigger than that of control cell. The extract of rosella flower at 0,5x104 μg/m has managed to lysis some MRSA bacterial cells and the cell wall of MRSA bacteria looks closely rapture, meanwhile the extract of rosella flower at 1x104 μg/m concentration is predicted to be able to lysis MRSA bacterial cell signed with the absence of MRSA growth in macroscopic observation.

Item Type: Thesis (Thesis)
Additional Information: KKA KK. TKT. 06-15 Swa e
Uncontrolled Keywords: Antibacterial activity, MBC, MIC, MRSA, SEM.s
Subjects: R Medicine
Divisions: 01. Fakultas Kedokteran > Ilmu Kedokteran Tropis
Creators:
CreatorsEmail
Swaidatul Masluhiya AF, 011214253010UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorAchmad Basori, Prof. Dr. Apt., MsUNSPECIFIED
ContributorKartuti Debora, dr., M.S., Sp.MK(K)UNSPECIFIED
Depositing User: Nn Deby Felnia
Date Deposited: 2016
Last Modified: 24 Oct 2016 17:04
URI: http://repository.unair.ac.id/id/eprint/33961
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