JUMLAH SEL LIMFOSIT PADA DAERAH PERI ARTERIAL LYMPHOID SHEATH (PALS) LIMPA DAN KADAR IL-2 MENCIT DIABETES MELLITUS (DM) DENGAN INDUKSI HISTAMIN INTRAPERITONEAL Suatu Penelitian Eksperimental Laboratorik pada Mencit strain BALB/c jantan

ARNI KUSUMA DEWI, NIM011041001 (2012) JUMLAH SEL LIMFOSIT PADA DAERAH PERI ARTERIAL LYMPHOID SHEATH (PALS) LIMPA DAN KADAR IL-2 MENCIT DIABETES MELLITUS (DM) DENGAN INDUKSI HISTAMIN INTRAPERITONEAL Suatu Penelitian Eksperimental Laboratorik pada Mencit strain BALB/c jantan. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background Diabetes Mellitus complication that much happen and need attention is diabetic ulcus. Bacterial infection in diabetic ulcus getting worse with the decrease of cellular immunity patient. Development of antibiotic as therapy of diabetic ulcus has not been optimaly yet without immunity intervention to the patient. Objective : This study is done to evaluate immunity state in diabetes with count lymphocyte in PALS spleen and measure Interleukin-2 cytokine in blood serum then compare both with healty ones. Material and Method : Thirty six mice BALB/c strain, sex male, 12 weeks years old, weight 25 – 30 g and healthy was into 2 groups : the control group and the treatment group. The control group had standart diet, while the treatment group had high fat diet with 22,8 % fat to induct diabetes for ten weeks. After that both groups were induced with histamine intraperitoneally with 10 mg/20 g BB dose to show immune respons. Mice were sacrified for taken the blood and the spleen. The blood serum were used for measuring level of IL-2 with ELISA reader, while the spleen were used for counting lymphocyte cell in the PALS area with made histology preparation H.E staining. Result : The IL-2 level of control and treatment both increased cause histamine induced, but the control was significant less than the treatment (p<0,0001). Account of lymphocyte cell in the PALS area shown that the control had significant more than the treatment (p<0,0001). Conclusion : the high level of IL-2 induced by high fat diet and the decrease of account of lymphocyte may caused by oksidatif stress in acute diabetes in obese mice.

Item Type: Thesis (Thesis)
Additional Information: KKA KK TKD.40/13 Dew j; KKB KK-2 TKD.40/13 De j; KKC KK TKD.40/13 Dew j
Uncontrolled Keywords: Diabetes Mellitus, BALB/c mice, Lymphocyte cell of spleen, level of IL-2
Subjects: R Medicine > RC Internal medicine > RC648-665 Diseases of the endocrine glands. Clinical endocrinology
R Medicine > RC Internal medicine > RC666-701 Diseases of the circulatory (Cardiovascular) system
S Agriculture > SF Animal culture > SF405.5-407 Laboratory animals
Divisions: 09. Sekolah Pasca Sarjana > Ilmu Kedokteran Dasar
Creators:
CreatorsNIM/NIDN
ARNI KUSUMA DEWI, NIM011041001NIM011041001
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorH. Ari Gunawan, Prof., dr., M.S., Ph.D., PA(K)UNSPECIFIED
ContributorIskantijah B. Rahardjo, dr., M.S., PA(K)UNSPECIFIED
Depositing User: Nn Duwi Prebriyuwati
Date Deposited: 2016
Last Modified: 06 Sep 2016 00:24
URI: http://repository.unair.ac.id/id/eprint/37136
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