IDENTIFIKASI PLASMODIUM KNOWLESI PADA PENDERITA MALARIA KLINIS DENGAN TEKNIK MIKROSKOPIS DAN POLYMERASE CHAIN REACTION DI KABUPATEN SERUYAN PROVINSI KALIMANTAN TENGAH

KASMIJATI, 011042003 (2012) IDENTIFIKASI PLASMODIUM KNOWLESI PADA PENDERITA MALARIA KLINIS DENGAN TEKNIK MIKROSKOPIS DAN POLYMERASE CHAIN REACTION DI KABUPATEN SERUYAN PROVINSI KALIMANTAN TENGAH. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Recently, Plasmodium knowlesi known to cause malaria in Macaca fascicularis monkey has been found to infect human in Southeast Asian. The first malaria knowlesi in human was reported from South Kalimantan Province, Indonesia, in 2010. However, it is difficult to differentiate P. knowlesi from other human Plasmodium by microscopy. The aim of this study was to identify P. knowlesi in clinical malaria patients by microscopy and polymerase chain reaction [PCR] technique, a sensitive and specific method for identifying species based on DNA, in Seruyan District, CentralKalimantan Province, Indonesia. Cross-sectional study was conducted in Hanau Hospital, Hanau Primary Health Centre [PHC] and Rantau Pulut I PHC at Surayan District, Middle Kalimantan Province from April to June 2012. Sixty four clinical malaria patients were recruited by informed consent. Plasmodium species was identified by microscopy and by nested PCR using set primers coding SSU rRNA gene. Human Plasmodium species identification by microcopy and by nested PCR resulted 29 [14] P. falciparum, 34 [41] P. vivax, and 1 [1] negative. Eight mixed infection, P. falciparum and P. vivax infection, was only detected by nested PCR. Total four P. knowlesi could be identified by nested PCR in microscopy samples of P. vivax [4 from 41] and was found in 6,3% [4] of total DNA samples [64]. These results showed that the nested PCR could identify Plasmodium spesies, including P. knowlesi, and knowlesi malaria may occur in Seruyan District, Middle Kalimantan Province. Based on the microscopy and nested PCR examination, it seemed that P. knowlesi may have a morphology and SSU rRNA gene similar with P. vivax. Therefore, it needs to further analysis for P. knowlesi identification by DNA sequensing and homology analysis with P. vivax gene

Item Type: Thesis (Thesis)
Additional Information: KKA KK TKT.05/13 Kas i; KKB KK-2 TKT.05/13 Kas i; KKC KK TKT.05/13 Kas i
Uncontrolled Keywords: Malaria, microscopic, PCR, Plasmodium falciparum, Plasmodium vivax, Plasmodium knowlesi
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
R Medicine > RC Internal medicine > RC109-216 Infectious and parasitic diseases
Divisions: 01. Fakultas Kedokteran > Ilmu Kedokteran Tropis
Creators:
CreatorsEmail
KASMIJATI, 011042003UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorYoes Prijatna Dachlan, Dr., dr., M.Sc.,Sp.ParKUNSPECIFIED
ContributorSukmawati Basuki, dr., M.ScUNSPECIFIED
Depositing User: Nn Duwi Prebriyuwati
Date Deposited: 2016
Last Modified: 06 Sep 2016 00:23
URI: http://repository.unair.ac.id/id/eprint/37141
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