PENENTUAN PROFIL PROTEIN BIOFILM Streptococcus mutans UNTUK EKSPLORASI BIOMARKER RISIKO KARIES GIGI

MALIKHATUN NI'MAH, 080942017 (2011) PENENTUAN PROFIL PROTEIN BIOFILM Streptococcus mutans UNTUK EKSPLORASI BIOMARKER RISIKO KARIES GIGI. Thesis thesis, UNIVERSITAS AIRLANGGA.

[img]
Preview
Text (ABSTRAK)
gdlhub-gdl-s2-2011-nimahmalik-21114-tk1411-k.pdf

Download (313kB) | Preview
[img] Text (FULL TEXT)
gdlhub-gdl-s2-2011-nimahmalik-17708-tk1411.pdf
Restricted to Registered users only

Download (1MB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

Biofilm consist of a collection of microorganisme witch attached to a surface and is covered by adhesive polysaccharides had excreted by these microorganisme. One of the negative influence of biofilm on human life is the emergence of dental caries. Streptococcus mutans is a virulent bacteria to biofilm formation causes dental caries. Dental caries can be several disease, including mediastinitis, sepsis, facial cellulitis, osteomyelitis, endocarditis, and pneumonia. Caries index in Indonesia around 2.2, caries index WHO’s target is 1.0. Alternative effort to suppress dental caries with early diagnostic of dental caries risk using biomarker. Biofilm growth performed of S. mutans on glass slide to BHIBS media for 24 hours. Crude protein extract of biofilm had obtained by lysis using ultrasonication 7 x 30s, 40 Hz. Furthermore, analysis antigenic protein biofilm S. mutans using Western blot methode. In this study also have done the determination protein profiles of S. mutans biofilm using SDS-PAGE 12%. Protein profile of S. mutans biofilm consist of seventeen bands, with known size 190, 185, 175, 157, 105, 85, 66, 55, 52, 42, 40, 29, 25, 22, 20,18, and 17 kDa. Antigenic protein of S. mutans biofilm consist of four bands, with known size 105, 52, 40, and 29 kDa.

Item Type: Thesis (Thesis)
Additional Information: KKC KK TK 14 / 11 Ni'm p
Uncontrolled Keywords: biofilm, ultrasonication, Western blot, SDS-PAGE, Streptococcus mutans
Subjects: Q Science > QD Chemistry > QD1-999 Chemistry
Q Science > QD Chemistry > QD241-441 Organic chemistry
Q Science > QR Microbiology > QR1-502 Microbiology
Divisions: 08. Fakultas Sains dan Teknologi > Program Studi Kimia (S2)
Creators:
CreatorsNIM/NIDN
MALIKHATUN NI'MAH, 080942017UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorAfaf Baktir, Dr.,MSUNSPECIFIED
ContributorIndah Listiana Kriswandini, Dr.,drg.,M.KesUNSPECIFIED
Depositing User: Nn Anisa Septiyo Ningtias
Date Deposited: 30 Jun 2016 04:08
Last Modified: 30 Jun 2016 04:08
URI: http://repository.unair.ac.id/id/eprint/38148
Sosial Share:

Actions (login required)

View Item View Item