METODE DILUSI AGAR MENGGUNAKAN MEDIUM KROMOGENIK MODIFIKASI FLUCONAZOLE UNTUK IDENTIFIKASI DAN PENENTUAN KEPEKAAN Candida Spp TERHADAP FLUCONAZOLE

PEPY DWI ENDRASWARI, 011141020 (2013) METODE DILUSI AGAR MENGGUNAKAN MEDIUM KROMOGENIK MODIFIKASI FLUCONAZOLE UNTUK IDENTIFIKASI DAN PENENTUAN KEPEKAAN Candida Spp TERHADAP FLUCONAZOLE. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Antifungal susceptibility testing is needed in the adequate management of candidiasis due to the increased of fluconazole resistance. Agar dilution method on chromogenic medium modified by fluconazole thought to be used as accurate and simple method. Purpose: To determine the accuracy of agar dilution method on chromogenic medium modified by fluconazole for species identification and fluconazole susceptibility testing in Candida spp. Method: The study design was observational study to study species identification and quasi experimental to study fluconazole susceptibility testing. Species identification and fluconazole susceptibility testing of Candida spp. were done by agar dilution method on chromogenic medium modified by fluconazole at concentrations of 0, 8, and 32 μg/ml and standard method, i.e.: API 20 C AUX and Vitek 2 compact. Accuracy analysis of agar dilution method on chromogenic medium modified by fluconazole was compared with standard method. Result: The results showed that of 25 isolates, the identification on chromogenic medium (CHROMagar Candida) obtained 12 isolates were C. albicans, 9 isolates were C. tropicalis, and 4 isolates were C. glabrata. The results showed 100% agreement to API 20 C AUX. Result of Fluconazole susceptibility testing showed that of the 25 isolates, obtained 21 isolates susceptible (S) by using both methods; 3 isolates susceptible (S) with Vitek 2 compact method and susceptible-dose dependent (S-DD) with a dilution method on chromogenic medium modified by fluconazole; 1 isolate resistant (R) by using both methods. Sensitivity, specificity, positive predictive value and negative predictive value of fluconazole susceptibility testing by agar dilution method on chromogenic medium modified by fluconazole was 100%. Conclusion: Agar dilution method on chromogenic medium modified by fluconazole showed a high accuracy for species identification and fluconazole susceptibility testing in Candida spp.

Item Type: Thesis (Thesis)
Additional Information: KKA KK TKD. 59/13 End m
Uncontrolled Keywords: agar dilution method, chromogenic medium, species identification, fluconazole susceptibility, Candida spp.
Subjects: Q Science > QD Chemistry > QD1-999 Chemistry
Q Science > QH Natural history > QH540-549.5 Ecology
Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions: 09. Sekolah Pasca Sarjana > Ilmu Kedokteran Dasar
Creators:
CreatorsEmail
PEPY DWI ENDRASWARI, 011141020UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorNi Made Mertaniasih, Prof. Dr., dr., MS., Sp.MK(K)UNSPECIFIED
ContributorBambang Susilo, dr., M.Kes., Sp.MK(K)UNSPECIFIED
Depositing User: Nn Duwi Prebriyuwati
Last Modified: 01 Sep 2016 07:12
URI: http://repository.unair.ac.id/id/eprint/38495
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