SENSITIVITAS DAN SPESIFISITAS MEDIA KROMOGENIK SEBAGAI DETEKSI DINI ESCHERICHIA COLI DAN KLEBSIELLA PNEUMONIAE PENGHASIL EXTENDED SPECTRUM ΒETA LACTAMASE (ESBL) DARI SPESIMEN URIN PASIEN DI RSUD DR. SOETOMO, SURABAYA

Cut Asmaul Husna, NIM011214153002 (2014) SENSITIVITAS DAN SPESIFISITAS MEDIA KROMOGENIK SEBAGAI DETEKSI DINI ESCHERICHIA COLI DAN KLEBSIELLA PNEUMONIAE PENGHASIL EXTENDED SPECTRUM ΒETA LACTAMASE (ESBL) DARI SPESIMEN URIN PASIEN DI RSUD DR. SOETOMO, SURABAYA. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The purpose of this study was to analyze the sensitivity and specificity of ESBL chromogenic media as the early detection and identification of ESBL producing E. coli and K. pneumoniae from urine specimen. This research is the validity of a diagnostic test. Total of 343 urine samples from pediatric�s room, internal medicine and surgery, all urine was inoculated on chromogenic medium, besides routine examination. The results that grown on chromogenic medium identified 98 samples based on colony color, 41 samples had pink burgundy colony and 28 samples produces green colony; while those grown on MCA and BA 146 samples were subsequently identified with the Phoenix, and obtained 32 samples E. coli and 18 samples ESBL-producing K.pneumoniae. The results were compared to count sensitivity and specificity, then the data were analyzed using Mc Nemar and Kappa test. Based on Fisher�s test for identification E. coli and K. pneumoniae was not found any significant difference between the sensitivity and specificity of a chromogenic medium with Phoenix, the sensitivity and specificity respectively for E. coli was 96.9% and 80%; and 100% for K. pneumoniae. The results of the detection and identification of ESBL-producing E. coli and K. pneumoniae were compared with the Phoenix showed P<, with a sensitivity of 98% and specificity of 85%. The conclusion is that the chromogenic medium can be used in the early detection and identification of ESBL-producing E. coli and K. pneumoniae in the urine specimen, which has a high sensitivity, but low specificity. The use of chromogenic medium in the identification of E. coli and K. pneumoniae directly on urine specimens showed sensitivity and specificity were higher in K. pneumoniae than E. coli.

Item Type: Thesis (Thesis)
Additional Information: KKA KK. TKD. 08-14 Hus s
Uncontrolled Keywords: Chromogenic medium, urine specimens, ESBL producing E. coli and K. pneumoniae , sensitivity and spesificity, Phoenix.
Subjects: R Medicine
Divisions: 09. Sekolah Pasca Sarjana > Ilmu Kedokteran Dasar
Creators:
CreatorsNIM/NIDN
Cut Asmaul Husna, NIM011214153002NIM011214153002
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorKuntaman, Prof. Dr., dr, MS., Sp.MK (K)UNSPECIFIED
ContributorKartuti Debora,, dr., MS., Sp.MK (K)UNSPECIFIED
Depositing User: Nn Deby Felnia
Date Deposited: 27 Oct 2016 18:15
Last Modified: 02 Jul 2017 17:46
URI: http://repository.unair.ac.id/id/eprint/39143
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