M. Anam Al-Arif, MP., Drh and Herman Setyono, MS., Drh and Tri Nurhajati, MS., Drh (2005) ISOLASI DAN KARAKTERISASI ENSIM SELULASE DARI KEONG EMAS DAN RAYAP SEBAGAI BAHAN PENDEGRADASI SELULOSA. UNIVERSITAS AIRLANGGA, -. (Unpublished)

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Abstract

The aim of this research is to know the type of celullase enzyme from golden snails gut and termites gut and to know the enzyme activity by in-vitro system. Supernatant from golden snails (Pomacea canaliculata) gut and termites (Macrotermes sp.) gut were characterized the enzymes type by SDS-PAGE, Western Blot and Dot Blot testing. The enzymes activity from golden snail and termites supernatant then tested by CMC, pNPC and pNPG. The result of this research were there are many protein bands on SDS-PAGE that estimate of cellulase enzyme type, that is exoglucanase, endoglucanase and fl¬glucocidase enzymes. Western Blot and Dot Blot test then used to ensure this estimation. Western Blot test didnt clear showed the protein bands, while Dot Blot test showed the antigen-antibody reaction from golden snail and termite cellulase same as purified cellulase from Trichoderma virile, that revealed exoglucanase, endoglucanase and 13-glucocidase enzymes. The golden snails endoglucanase activity was higher than termites endoglucanase activity. The golden snails exoglucanase activity was higher than termites exoglucanase activity. The golden snails [3-glucocidase activity was higher than termites 3-glucocidase activity. The conclusion of this research were there are 3 cellulase enzymes type in golden snail and termite gut, that is exoglucanase, endoglucanase and ll-glucocidase enzymes. The golden snails cellulase enzymes activity was higher than termites cellulase enzymes activity.

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