ISOLASI DAN KARAKTERISASI ENZIM β-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. capitata L.)

Manuhara, Yosephine Sri Wulan (2010) ISOLASI DAN KARAKTERISASI ENZIM β-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. capitata L.). Berkala Penelitian HAYATI(Journal of Biological Researches)., 15 (2). pp. 99-104. ISSN 0852-6834

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ISOLASI DAN KARAKTERISASI ENZIM β-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv - Bukti C07 - Isolasi dan Karakterisasi Enzim b-1,3-endoglukanase.pdf

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Abstract

Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40oC of optimum temperature, and optimum pH is 7. After the purification with hydrophobic interaction chromatography and ion exchange chromatography, it’s activity was increased. Based on SDS-PAGE analysis, β-1,3-endoglucanase have molecular weight around 48 kD. Antifungal activity of β-1,3-endoglukanase show that it has best inhibition zone on Fusarium solanii at extract from ion exchange chromatography.

Item Type: Article
Uncontrolled Keywords: isolation, characterization, β-1,3-endoglucanase, Brassica oleracea var. capitata
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH540-549.5 Ecology
Q Science > QK Botany > QK900-989 Plant ecology
S Agriculture
Divisions: 08. Fakultas Sains dan Teknologi > Biologi
Creators:
CreatorsNIM/NIDN
Manuhara, Yosephine Sri WulanUNSPECIFIED
Depositing User: Mr Akhmad Nurfizal Reza
Date Deposited: 13 Nov 2016 16:50
Last Modified: 13 Nov 2016 16:50
URI: http://repository.unair.ac.id/id/eprint/45565
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