THE EXPRESSION OF TLR-2 AND NOD-2 IN GINGIVAL EPITHELIUM OF RAT AFTER PROBIOTIC Lactobacillus reuteri SUPPLEMENTATION TO INHIBIT Streptococcus mutans GROWTH

Kusumaningsih, Tuti and Sudarmo, Subijanto Marto and Indrawati, Retno and Ridwan, Rini Devijanti and Sidarningsih, - (2015) THE EXPRESSION OF TLR-2 AND NOD-2 IN GINGIVAL EPITHELIUM OF RAT AFTER PROBIOTIC Lactobacillus reuteri SUPPLEMENTATION TO INHIBIT Streptococcus mutans GROWTH. Journal of BIOLOGICAL RESEARCHES, 21 (1). ISSN 08526834

[img] Text
867-2432-1-SM.pdf
Restricted to Registered users only

Download (574kB)
[img]
Preview
Text
tuti 9.pdf

Download (1MB) | Preview
Official URL: http://berkalahayati.org

Abstract

Lactobacillus reuteri is probiotic from Gram positive bacteria which has specific molecular structure, consisted of peptidoglycan (PG) and lipotheihoic acid (LTA). These structure have potential in pattern recognition receptors (PRRs) activation, such as TLR-2 and NOD-2 that are the up stream of beta defensin-2 (BD-2) signaling cascade. BD-2 is antimocrobial peptides naturally produced in mouth cavity that can against Streptococcus mutans effectively. This study was aimed to prove that probiotic L. reuteri supplementation can increase the expression of TLR-2 and NOD-2 in gingival epithelium. Experimental design in this study was randomized control group post test only design. Study was carried on 24 white rat (Rattus norvegicus) Wistar strain which divided into 4 groups. Positive control was rats that induced with S. mutans, while rats in negative control group were not induced. Group I was rats that suppelemented with L. reuteri for 14 days (day 1-14) and induced with S. mutans for 7 days (day 8-14). Group II was rats that supplemented with L. reuteri and induced with S. mutans simustaneously for 14 days (day 1-14). Concentration of bacterial suspension was 108 cfu ∕ml for L. reuteri and 1010 cfu/ml for S. mutans. Both of these two bacteria was given orally to rats. TLR-2 and NOD-2 expressions were evaluated with immunohistochemistry technique. Significant differences of protein expression between each treatment groud was analyzed with ANOVA (p=0.001). TLR-2 nd NOD-2 expressions were higher than negative control. It can be conclude that L. reuteri supplementation as probiotic could increase the expression of TLR-2 and NOD-2 in gingival epithelium of rat

Item Type: Article
Uncontrolled Keywords: Probiotic, L. reuteri, TLR-2,NOD-2, caries, S. mutans
Subjects: Q Science > Q Science (General)
Q Science > Q Science (General) > Q1-295 General
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi
Peer Review
Creators:
CreatorsEmail
Kusumaningsih, TutiUNSPECIFIED
Sudarmo, Subijanto MartoUNSPECIFIED
Indrawati, RetnoUNSPECIFIED
Ridwan, Rini DevijantiUNSPECIFIED
Sidarningsih, -UNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 06 Dec 2016 19:12
Last Modified: 06 Dec 2016 19:12
URI: http://repository.unair.ac.id/id/eprint/47895
Sosial Share:

Actions (login required)

View Item View Item