Cytotoxicity Test of Amethyst Root Extract (Datura metel L.) on Fibroblast Cell Using MTT Assay

Kusumaningsih, Tuti and Pratiwi, Fitri Dwi Agus and Sunariani, Jenny (2015) Cytotoxicity Test of Amethyst Root Extract (Datura metel L.) on Fibroblast Cell Using MTT Assay. Oral Biology Dental Journal, 7 (1). pp. 38-43. ISSN 1979-8814

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Official URL: http://dentj.fkg.unair.ac.id/cytotoxicity-test-of-...

Abstract

Background. Antibiotic resistance could occur due to excessive use of antibiotics or using antibiotics without supervision of a doctor. Resistance to certain antibiotics may cause some failure in treatment of infectious disease. Root of amethyst contains antibacterial contents named flavonoid, tannin and saponin. Natural ingredients do not cause toxic effects, but scientific studies have not been performed to prove that the natural material is not toxic. Purpose. The aim of this study is to determine the toxicological properties of amethyst root extract on fibroblast BHK-21. Methods. Microplate containing fibroblasts BHK-21 cells that had been exposed to three concentration of amethyst root extract (10%,17,5% and 25%) was incubated 5% CO2 at a temperature of 37º C in the incubator for 20 hours. The results of MTT essay can be seen from the absorbent solution formazan crystals through specific wavelength 620 nm with elisa reader. Results. Number of fibroblasts BHK-21 which alives after the exposure of amethyst root extract on each three concentrations shows that the viability is above 50%. Conclusion. Amethyst root extract (Datura metel L.) is not toxic against fibroblasts. Keywords: Datura metel L, cytotoxicity test, fibroblast BHK-21, MTT assay

Item Type: Article
Uncontrolled Keywords: Datura metel L, cytotoxicity test, fibroblast BHK-21, MTT assay,
Subjects: Q Science > Q Science (General)
Q Science > Q Science (General) > Q1-295 General
R Medicine > RK Dentistry > RK1-715 Dentistry
Divisions: Peer Review
02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsEmail
Kusumaningsih, TutiUNSPECIFIED
Pratiwi, Fitri Dwi AgusUNSPECIFIED
Sunariani, JennyUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 14 Dec 2016 20:28
Last Modified: 18 Jan 2017 19:54
URI: http://repository.unair.ac.id/id/eprint/48626
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