POVIDONE IODINE TO INHIBIT THE GROWTH OF BACTERIA

DAYANARA PRAMA ISWARI, NIM011211133102 (2016) POVIDONE IODINE TO INHIBIT THE GROWTH OF BACTERIA. Skripsi thesis, Universitas Airlangga.

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Abstract

Introduction: The awareness of keeping a good hygiene is increasing these days. One of the mostimportant parts of keeping a good hygiene is by utilizing several antiseptics or antibacterial agents. One of which is iodine, which has a microbicide action against most of the important healthrelated microorganism. Povidone, a popular carrier for iodine, slowly and continuously release free iodine into solution, maintaining the microbicidal activity for a long period of time and decreases the toxicity. The efficacy of Povidone iodine on different concentrations is still questionable; some bacteria like Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa may even develop resistance towards it. Through this study, the author wants to explain the efficacy of povidone iodine as one of the antimicrobial agents to prevent growth of aforementioned bacteria. Methods: This research was conducted through a true experimental laboratory study by applyingvarious MICs. The 1st group consists of 3 tubes with Mueler-Hinton broth containing Staphylococcus aureus, which was given 10%, 5%, and 2.5% of povidone iodine respectively. The 1st group consists of 3 tubes with Mueler-Hinton broth containing Escherichia coli, which was given 10%, 5%, and 2.5% of povidone iodine respectively. The 1st group consists of 3 tubes with Mueler- Hinton broth containing Pseudomonas aeruginosa, which was given 10%, 5%, and 2.5% of povidone iodine respectively. Each group has an extra tube with only a nutrient agar and the selected bacteria, which acts as a positive control. After 24 hours of incubation, every tube from each group are smeared to an agar to be examined. Results: All of the smears of Povidone iodine containing tubes, showed no growth of bacteria, whilein contrast, the growth of bacteria in control positive plates are positive. Conclusion: 2.5% of concentration of Povidone iodine alone is enough to successfully inhibit thegrowth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa with no sign of bacterial resistance towards Povidone iodine.

Item Type: Thesis (Skripsi)
Additional Information: KKA KK FK.PD.12-16sw p
Uncontrolled Keywords: POVIDONE IODINE; BACTERIA
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions: 01. Fakultas Kedokteran
Creators:
CreatorsNIM
DAYANARA PRAMA ISWARI, NIM011211133102NIM011211133102
Contributors:
ContributionNameNIDN / NIDK
Thesis advisorAgung Dwi Wahyu Widodo, Dr., dr. MsiUNSPECIFIED
Thesis advisorSri Purwaningsih, dr., M. KesUNSPECIFIED
Depositing User: Tatik Poedjijarti
Date Deposited: 30 Jan 2017 20:55
Last Modified: 30 Jan 2017 20:55
URI: http://repository.unair.ac.id/id/eprint/52159
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