Narumi, Hasutji Endah and Chusniati, Sri (2008) Isolasi dan Berat Melekul Protein OMP Kuman Salmonella Pullorum Isolat Lapangan. Veterinary Anatomy Journal, 1 (2). pp. 57-60. ISSN 1979-7249

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Abstract

Pullorum disease use to be called Bacillary white diarrhoea disease and it is one of contagious bacterial disease in Poultry. The disease is an acute and it is highly fatal to young chicks during the first few day of life. In the adult there may be also be chronic cases and it is changeable as carrier. This research was done in 4 steps: (1) isolation, identification and purification of Salmonella pullorum field isolate, (2) isolate of Salmonella pullorum Outer Membrane Protein (OMP), (3) measurement of OMP and (4) measurement weight molecule of OMP. The result showed that isolation, identification and purification from faeces of chick was pure Salmonella pullorum. The soluble OMP was found 20 ml (1973,4 ug/ml). the molecule weight were showed by the band on SDS-Page: 40 kDa, 29 kDa, 25 kDa, 19 kDa, 9 kDa, 4 kDa and 1 kDa. The measurement of molecule weight used standard marker (2, 4, 6, 14, 21, 30 and 46 kDa) according to Wolfgang et al (1992), the molecule of antigenically Gram negative was 20 to 45 kDa, where as molecule weight of Escherichia coli protein was around 35 kDa. Salmonella pullorum , Outer Membrane Protein, SDS-PAGE

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