Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent

Nike Hendrijantini, NIDN. 0006105908 and Utari Kresnoadi, NIDN. 0011015403 and Sherman Salim, NIDN. 8833370018 and Bambang Agustono, NIDN. 0027086304 and Endang Retnowati, NIDN. 071608640 and Iwan Syahrial and Pungky Mulawardhana, NIDN. 0003068107 and Manggala Pasca Wardhana, NIDN. 0020118404 and Coen Pramono, NIDN. 0010025405 and Fedik Abdul Rantam, NIDN. 0010035907 (2015) Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells(hUCMSCs) with Gelatin Solvent. Journal Biomedical Science and Engineering, 8 (7). pp. 420-428. ISSN 1937-6871

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Abstract

The human umbilical cord is a source of numerous Mesenchymal Stem Cells (MSCs), making it as a potential source of allogeneic multipotent cell for bone tissue engineering. The aims of this study were to find: 1) Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) phenotypic characterization, 2) The in-vitro osteogenic differentiation potential of hUCMSCs, 3) The cytotoxicity of gelatin solvent to hUCMSCs using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay. As a result, through characterization of hUCMSCs, the majority of target cells expressed specific MSCs markers, Cellular Differentiation (CD)73, smaller number of subpopulation expressed CD90 with only minimal subpopulation expressed CD105 and all negative MSCs markers. Osteoblastic differentiation was found in a significantly high number of cells when in vitro osteogenic differentiation of hUCMSCs with Alizarin Red staining was done. Biocompatibility analysis using the MTT assay showed that gelatin solvent and Alpha modification of minimum essential

Item Type: Article
Uncontrolled Keywords: Human Umbilical Cord Mesenchymal Stem Cells, Mesenchymal Stem Cells, Phenotypic Characterization, Osteogenic Differentiation, Bone Tissue Engineering
Subjects: R Medicine
R Medicine > RK Dentistry
R Medicine > RK Dentistry > RK641-667 Prosthetic dentistry. Prosthodontics
Divisions: 02. Fakultas Kedokteran Gigi
Peer Review
Creators:
CreatorsNIM/NIDN
Nike Hendrijantini, NIDN. 0006105908UNSPECIFIED
Utari Kresnoadi, NIDN. 0011015403UNSPECIFIED
Sherman Salim, NIDN. 8833370018UNSPECIFIED
Bambang Agustono, NIDN. 0027086304UNSPECIFIED
Endang Retnowati, NIDN. 071608640UNSPECIFIED
Iwan SyahrialUNSPECIFIED
Pungky Mulawardhana, NIDN. 0003068107UNSPECIFIED
Manggala Pasca Wardhana, NIDN. 0020118404UNSPECIFIED
Coen Pramono, NIDN. 0010025405UNSPECIFIED
Fedik Abdul Rantam, NIDN. 0010035907UNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 15 Mar 2017 19:58
Last Modified: 23 Sep 2019 02:47
URI: http://repository.unair.ac.id/id/eprint/54565
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