FAISAL ANDRIANTO, 061111091
(2016)
PENGARUH SARI KULIT DAN BUAH SEMANGKA MERAH
(Citrullus lanatus) SEBAGAI BAHAN PENGENCER
TERHADAP MOTILITAS DAN VIABILITAS
SPERMATOZOA DOMBA.
Skripsi thesis, Universitas Airlangga.
Abstract
Aim of this research was to study the effect of peel and flesh of
watermelon as diluter on motility and viability of ram spermatozoa. There were
three treatments: 1) KTS= egg yolk citrate; 2) BSS= watermelon flesh extract
citrate; 3) P3= watermelon peel extract citrate. Each treatments were stored at 5oC
and observed for five level of storage time, which were day 1, day 2, day 3, day 4,
and day 5. Data were analysed using completely randomised design with factorial
pattern 3x5 followed by duncan multiple range test if there were significant
differences (P<0.05). Result showed there was interaction between treatments and
storage times which were significantly different (P<0,05) towards motility and
viability of spermatozoa. The highest percentage of motility and viability were
obtained from BSS for 1 day of storage which were 76.25±4.787% and
82.75±3.304%. The lowest percentage of motility and viability were obtained
from KSS for 5 days of storage which were 23.75±2.062% and 31.75±2.363%.
From the overall storage time, the average spermatozoa motility and viability’s
percentage of KTS showed the best result which were 58.00±13.183% and
66.00±10.959%. Conclusion of this research was the extract of watermelon peel
and flesh can be used as a diluter only for four days of storage time. Therefore
addition of other substance which can protect spermatozoa from cold shock was
needed to extend storage time more than four days.
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