The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers

D.M. Hariyadi and Y. Ma and Y. Wang and T. Bostrom and J. Malouf and M.S. Turner and B. Bhandari and A.G.A. Coombes (2014) The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers. The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers, 24 (2). pp. 178-184. ISSN ISSN-1773-2247

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Official URL: http://www.journals.elsevier.com/journal-of-drug-d...

Abstract

Oral administration of dry vaccine formulations is acknowledged to offer major clinical and logistical benefits by eliminating the cold chain required for liquid preparations. A model antigen, bovine serum albumin (BSA) was encapsulated in alginate microspheres using aerosolisation. Hydrated microspheres 25 to 65 µm in size with protein loading of 3.3 % w/w were obtained. Environmental scanning electron microscopy indicated a stabilizing effect of encapsulated protein on alginate hydrogels revealed by an increase in dehydration resistance. Freeze drying of alginate microspheres without use of a cryoprotectant resulted in fragmentation and subsequent rapid loss of the majority of the protein load in simulated intestinal fluid in 2 h, whereas intact microspheres were observed following freeze-drying of BSA-loaded microspheres in the presence of maltodextrin. BSA release from freeze-dried preparations was limited to less than 7 % in simulated gastric fluid over 2 h, while 90 % of the protein load was gradually released in simulated intestinal fluid over 10 h. SDS-PAGE analysis indicated that released BSA largely preserved its molecular weight. These findings demonstrate the potential for manufacturing freeze-dried oral vaccines using alginate microspheres.

Item Type: Article
Uncontrolled Keywords: Oral vaccine – Alginate hydrogel microspheres – Maltodextrin cryoprotectant – Protein – Bovine serum albumin – ESEM – In vitro release
Subjects: R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS200-201 Pharmaceutical dosage forms
Divisions: 05. Fakultas Farmasi > Farmastika
Creators:
CreatorsNIM/NIDN
D.M. Hariyadidewi-m-h@ff.unair.ac.id
Y. MaUNSPECIFIED
Y. WangUNSPECIFIED
T. BostromUNSPECIFIED
J. MaloufUNSPECIFIED
M.S. TurnerUNSPECIFIED
B. BhandariUNSPECIFIED
A.G.A. CoombesUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 06 Apr 2017 19:35
Last Modified: 17 May 2017 16:14
URI: http://repository.unair.ac.id/id/eprint/56080
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