CANINDERA COSTA, 051211131071
(2016)
UJI AKTIVITAS ANALGESIK SENYAWA 4-BROMOBENZOILUREA PADA MENCIT PUTIH (Mus musculus) DENGAN METODE WRITHING TEST.
Skripsi thesis, Universitas Airlangga.
Abstract
The Analgesic Activity Test of 4-Bromobenzoylurea Compound in Mice
(Mus musculus) Using Writhing Test Method
The research purposed to synthesize 4-Bromobenzoylurea and to
determine its analgesic activity in mice (Mus musculus) using writhing test
method was done. The Schotten-Baumann reaction was used for reaction
between 4-bromobenzoylchloride and urea with adding trietilamine base. It
could be concluded that the product was pure from the result of Thin Layer
Chromatography and melting point analysis. According to the result of
infrared and ultraviolet spectrophotometric analysis, it was concluded that
the synthesized compound was 4-bromobenzoylurea. The analgesic activity
was tested using writhing test method in three different doses 50, 100 and
200 mg/kg mice body-weight. The result of analgesic activity test was
writhing frequency, which was analyzed to determine pain-inhibition
percentage and then ED50. The pain-inhibition percentage of 4-
bromobenzoylurea, benzoylurea, and acetic salicylic acid compound, each
in three different doses was 22,25%; 44,74%; 56,46%; 21,77; 43,78%;
54,07% and 32,78%; 58,13%; 77,51%. The ED50 of 4-bromobenzoylurea,
benzoylurea, and acetic salicylic acid was 143,14; 154,54 and 82,70 mg/kg
mice body-weight. According to the result of writhing frequency one-way
analysis of variance and LSD test, it could be concluded that the 4-
bromobenzoylurea compound has the analgesic activity, that as strong as
benzoylurea compound but weaker than acetic salicylic acid analgesic
activity.
Keyword : 4-Bromobenzoylurea, Benzoylurea, Writhing test.
Actions (login required)
 |
View Item |
