PENGARUH KONSENTRASI BUNGKIL INTI SAWIT TERHADAP AKTIVITAS MANANASE HASIL FERMENTASI Bacillus subtilis ATCC 6633

CASHIN JAPLIN, 051211132032 (2016) PENGARUH KONSENTRASI BUNGKIL INTI SAWIT TERHADAP AKTIVITAS MANANASE HASIL FERMENTASI Bacillus subtilis ATCC 6633. Skripsi thesis, Universitas Airlangga.

[img]
Preview
Text (abstrak)
FF. KF. 39-16 Jap p abstrak.pdf

Download (407kB) | Preview
[img] Text (fulltext)
FF. KF. 39-16 Jap p.pdf
Restricted to Registered users only

Download (2MB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

Influence of Palm Kernel Cake Concentration To Mannanase Activi ty From Bacillus subtilis ATCC 6633 Mannanase is an hydrolytic enzyme that can hydrolize both the polysaccharide mannan and manno-oligosaccharides linked by a β-1,4 mannosidic bond. Mannanase can be applied to animal feed industry, paper, pharmacy, food, detergent and oil and gas. Plants and microorganisms especially bacteria Gram positive like Bacillus subtilis can produce mannanase by fermentation process using palm kernel cake (PKC) as a substrate. In this research, a Bacillus subtilis ATCC 6633 used as mannanase resource fermented in agar media containing PKC. This research was carried out to find the ability of Bacillus subtilis ATCC 6633 to produce mannanase by fermentation process using PKC as a subtrate and concentration of PKC which can produce maximum mannanase activity. The research is conducted in several steps, as follows: reduction of PKC particle size, inoculation of Bacillus subtilis ATCC 6633, growth test of Bacillus subtilis ATCC 6633 and act ivity test of mannanase using locust bean gum (LBG) and PKC as a subtrate. The fermentation was performed using agar-well difusion method at 120 hours and 37 °C.The results showed that mannanase activity appears as clear zone obtained around the bacterial colonies. Diameter of clear zone is converted into mannanolytic index. Mannanase activity at 0,5% LBG produced mannanolytic index of 2,6 ± 0,22 and 0,25% BIS produced mannanolytic index of 4,0 ± 0,98. Keywords : Mannanase, Enzyme, Mannan, Bacillus subtilis ATCC 6633, Palm kernel cake

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF. KF. 39-16 Jap p
Uncontrolled Keywords: Mannanase, Enzyme, Mannan, Bacillus subtilis ATCC 6633, Palm kernel cake
Subjects: R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Kimia Farmasi
Creators:
CreatorsEmail
CASHIN JAPLIN, 051211132032UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorIsnaeni, Dr., Apt., MS.,UNSPECIFIED
ContributorAchmad Toto Poernomo, Drs., Apt., M.Si.,UNSPECIFIED
Depositing User: Tatik Poedjijarti
Date Deposited: 10 Apr 2017 16:37
Last Modified: 10 Apr 2017 16:37
URI: http://repository.unair.ac.id/id/eprint/56222
Sosial Share:

Actions (login required)

View Item View Item