Andrographolide determination of Andrographis paniculata extracts, ethyl acetate fractions and tablets by thin layer chromatography

Achmad, Fuad Hafid and Bachtiar, Rifai and Lidya, Tumewu and Endang, Widiastuti and Lilis, Dachliyati and Riesta, Primaharinastiti and Aty, Widyawaruyanti (2015) Andrographolide determination of Andrographis paniculata extracts, ethyl acetate fractions and tablets by thin layer chromatography. Journal of Chemical and Pharmaceutical Research, 7. pp. 557-561. ISSN 0975-7384

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Official URL: http://www.jocpr.com/archive/jocpr-volume-7-issue-...

Abstract

The purpose of this study was to develop a simple, sensitive and validated analytical method to determine andrographolide in Andrographis paniculata. Simple Thin Layer Chromatograpy (TLC) method was performed to determine andrographolide in extracts, ethyl acetate fractions and tablets of A. paniculata with silica gel 60 F254 as stationary phase. Linear ascending development was carried out with chloroform: methanol (9:1 v/v). The Rf value of andrographolide was found to be 0.39. Camag TLC Scanner 3 was used for peak analysis and scanned at wavelenght 228 nm. The correlation coefficient of the calibration curve was 0.998 in the range of 0.08 µg to 0.60 µg. The recovery of the method was 106.74% - 109.72%. The relative standard deviation was 1.52 - 4.48%. Application this method for andrographolide determination in sample of phytopharmaceutical product development showed andrographolide content in 96% ethanol extract, 70% ethanol extract, ethyl acetate fraction-96, ethyl acetate fraction-70, ethyl acetate fraction-96 tablet and ethyl acetate fraction-70 tablet were 13.94%, 10.12%; 27.22%, 33.12%, 6.51% and 6.10%, respectively. The proposed analytical method was simple, selective and valid for determination of andrographolide in extracts, ethyl acetate fractions and tablets of A. paniculata.

Item Type: Article
Uncontrolled Keywords: TLC, andrographolide, A. paniculata, extract, ethyl acetate fraction, tablet.
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Farmakognosi Fitokimia
05. Fakultas Farmasi > Kimia Farmasi
Creators:
CreatorsNIM/NIDN
Achmad, Fuad Hafidachmadfuad@ff.unair.ac.id
Bachtiar, RifaiUNSPECIFIED
Lidya, TumewuUNSPECIFIED
Endang, WidiastutiUNSPECIFIED
Lilis, DachliyatiUNSPECIFIED
Riesta, Primaharinastitiriesta-p-h@ff.unair.ac.id
Aty, Widyawaruyantiaty-w@ff.unair.ac.id
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 08 May 2017 21:36
Last Modified: 08 May 2017 21:36
URI: http://repository.unair.ac.id/id/eprint/56572
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