Activities of Ficus fistulosa Leave Extract and Fractions Against Hepatitis C Virus

Achmad, Fuad Hafid and Adita, Ayu Permanasari and Lidya, Tumewu and Myrna, Adianti and Chie, Aoki and Aty, Widyawaruyanti and Soetjipto, Soetjipto and Maria, Inge Lusida and Hak, Hotta (2016) Activities of Ficus fistulosa Leave Extract and Fractions Against Hepatitis C Virus. Procedia Chemistry, 18. pp. 179-184. ISSN 18766196

[img] Text (FULLTEXT)
1-s2.0-S1876619616000292-main.pdf__tid=26a71966-24a5-11e7-b3fa-00000aacb35f&acdnat=1492567919_334e9af4cbacb32926330261525870dd
Restricted to Registered users only

Download (308kB) | Request a copy
[img] Text (Peer Review)
12 Penilaian Reviewer.pdf
Restricted to Registered users only

Download (1MB) | Request a copy
Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

Hepatitis C Virus (HCV) is a major global disease which often leads to chronicity and is potential to liver failure. There is no anti-HCV vaccine and the high diversity of viral genotypes will probably make it very difficult to develop a vaccine. Therefore, the development of new drugs for HCV treatment is highly required. It is commonly known that numerous important modern drugs have been developed from molecules originally isolated from natural sources. In this study, we tested the leave extract and fractions of Ficus fistulosa for their anti-HCV activities by cell culture method using Huh7it cells and HCV JFH1a. The result showed that ethanol extract of Ficus fistulosa (FFL) inhibited HCV JFH1a with IC50 value of 20.43±4.51 μg/ml. Toxicity test also indicated that FFL was not toxic with CC50 value of >200 μg/ml. The extract was further fractionated using chloroform (FFLC) and butanol (FFLB) successively. FFLC showed anti-HCV activity with IC50 value of 5.67±1.54 μg/ml and CC50 value of >100 μg/ml (Selectivity index >17.65). Further separation of FFLC by open column chromatography resulted in 12 subfractions (FFLC1-C12). Two subfractions, FFLC10, and FFLC11 showed high selectivity index (>100) with IC50 value of 0.60±0.30 μg/ml and 0.43±0.29 μg/ml, respectively. Therefore the leave extract (FFL) and fractions (FFL10, FFL11) of Ficus fistulosa would be a good candidate to develop antiviral against HCV.

Item Type: Article
Uncontrolled Keywords: Hepatitis C Virus; antiviral activity; Ficus fistulosa
Subjects: Q Science > QR Microbiology > QR355 Virology
R Medicine
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Farmakognosi Fitokimia
Creators:
CreatorsNIM/NIDN
Achmad, Fuad Hafidachmadfuad@ff.unair.ac.id
Adita, Ayu PermanasariUNSPECIFIED
Lidya, TumewuUNSPECIFIED
Myrna, AdiantiUNSPECIFIED
Chie, AokiUNSPECIFIED
Aty, Widyawaruyantiaty-w@ff.unair.ac.id
Soetjipto, SoetjiptoUNSPECIFIED
Maria, Inge LusidaUNSPECIFIED
Hak, HottaUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 08 May 2017 21:39
Last Modified: 08 May 2017 21:39
URI: http://repository.unair.ac.id/id/eprint/56583
Sosial Share:

Actions (login required)

View Item View Item