The Role Of Ethyl Acetate Fraction Of Andrographis Paniculata And Doxorubicin Combination Toward The Increase Of Apoptosis And Decrease Of VEGF Protein Expression Of Mice Fibrosarcoma Cells

Sukardiman, NIDN. 0001096305 and Suharjono and Triana Balqianur (2015) The Role Of Ethyl Acetate Fraction Of Andrographis Paniculata And Doxorubicin Combination Toward The Increase Of Apoptosis And Decrease Of VEGF Protein Expression Of Mice Fibrosarcoma Cells. International Journal of Pharmacy and Pharmaceutical Sciences, 7 (4). pp. 347-350. ISSN 0975–1491

[img] Text (FULLTEXT)
4838-18253-1-PB.pdf
Restricted to Registered users only

Download (393kB) | Request a copy
[img] Text (Peer Review)
5. Bukti C-05 Penilaian Karya Ilmiah.pdf
Restricted to Registered users only

Download (794kB) | Request a copy
Official URL: https://innovareacademics.in/journals/index.php/ij...

Abstract

Objective: The objective of this study is to prove the role of an ethyl acetate fraction of Andrographis paniculata and doxorubicin combination toward the increase of apoptosis and decrease of VEGF protein expression of mice fibrosarcoma cells. Methods: The mice were injected subcutaneously on the scapular with benzo(a)pyrene once every 2 days for 10 days to induce fibrosarcoma and after 3 months they were randomly divided into four groups, consisting of: negative control, ethyl acetate fraction of A. paniculata group, doxorubicin group, and combination of ethyl acetate fraction of A. paniculata and doxorubicin group. By immunohistochemistry preparation, fibrosarcoma cells were scored for apoptosis and VEGF protein expression. Results: Ethyl acetate fraction of A. paniculata and doxorubicin combination significantly (p<0.05) increase apoptosis. Ethyl acetate fraction of A. paniculata and doxorubicin combination also significantly (p<0.05) decrease VEGF protein expression. Conclusion: It can be concluded that ethyl acetate fraction of A. paniculata and doxorubicin combination increase apoptosis and decrease VEGF protein expression of mice fibrosarcoma cells. Decrease of VEGF protein expression led to an inhibition of tumor growth, tumor neovascularization, decrease in proliferation of tumor cells and the formation of necrosis.

Item Type: Article
Uncontrolled Keywords: Andrographis paniculata, Doxorubicin, Apoptosis, VEGF, Fibrosarcoma, Benzo(a)pyrene.
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Farmakognosi Fitokimia
05. Fakultas Farmasi > Farmasi Klinis
Peer Review
Creators:
CreatorsNIM/NIDN
Sukardiman, NIDN. 0001096305sukardiman@ff.unair.ac.id
Suharjonosuharjono@ff.unair.ac.id
Triana BalqianurUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 24 Apr 2017 19:06
Last Modified: 24 Apr 2017 19:06
URI: http://repository.unair.ac.id/id/eprint/56669
Sosial Share:

Actions (login required)

View Item View Item